Fusion of Oligopeptide to the C Terminus of α-Glucuronidase from Thermotoga maritima Improves the Catalytic Efficiency for Hemicellulose Biotransformation

Autor:	Hongyang Zhao, Yemin Xue, Mengke Xue, Fang Xie, Yaxian Li, Zixuan Ding
Rok vydání:	2022
Předmět:	Bioengineering Molecular Biology Applied Microbiology and Biotechnology Biochemistry Biotechnology
Zdroj:	Molecular Biotechnology. 65:741-751
ISSN:	1559-0305 1073-6085
DOI:	10.1007/s12033-022-00569-y
Popis:	Fusion protein combined the oligopeptide (HQAFFHA) with the C terminus of α-glucuronidase from Thermotoga maritima was produced in E. coli and purified for characterization and applications of glucuronic and glucaric acid production. The fusion protein with oligopeptide exhibited a 2.97-fold higher specific activity than individual protein. Their catalytic efficiency k
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::819d6827538c2469234d65ffab7a9f2e https://doi.org/10.1007/s12033-022-00569-y Zobrazit plný text záznamu Full text from SpringerLink