Bacterial Metabolism of para - and meta -Xylene: Oxidation of a Methyl Substituent
| Autor: | John F. Davey, David T. Gibson |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Chemical Phenomena
Stereochemistry Physiology and Metabolism Catechols Substituent Microbial metabolism Alcohol oxidoreductase Alcohol Xylenes Biology Benzoates Methylation Microbiology Catalysis Cell-free system chemistry.chemical_compound Pseudomonas Molecular Biology Nitrosoguanidines Cell-Free System Succinates biology.organism_classification Aldehyde Oxidoreductases Toluene Alcohol Oxidoreductases Chemistry chemistry Biochemistry Mutation Oxygenases Oxidation-Reduction Mutagens |
| Zdroj: | Journal of Bacteriology. 119:923-929 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.119.3.923-929.1974 |
| Popis: | Pseudomonas Pxy was isolated on p -xylene as sole source of carbon and energy. Substrates that supported growth were toluene, p -methylbenzyl alcohol, p -tolualdehyde, p -toluic acid, and the analogous m -methyl derivatives, including m -xylene. Cell extracts prepared from Pseudomonas Pxy after growth with either p -xylene or m -xylene oxidized the p - and m -isomers of tolualdehyde as well as p -methylbenzyl alcohol. The same cell extracts also catalyzed a “meta” fission of both 3- and 4-methylcatechol. Treatment of Pseudomonas Pxy with N -methyl- N ′-nitro- N -nitrosoguanidine led to the isolation of two mutant strains. Pseudomonas Pxy-40, when grown on succinate in the presence of p -xylene, accumulated p -toluic acid in the culture medium. Under the same conditions Pseudomonas Pxy-82 accumulated p -toluic acid and also 4-methylcatechol. When Pseudomonas Pxy-82 was grown on succinate in the presence of m -xylene, 3-methylcatechol and 3-methylsalicylic acid were excreted into the culture medium. A pathway is proposed for the initial reactions utilized by Pseudomonas Pxy to oxidize p - and m -xylene. |
| Databáze: | OpenAIRE |
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