Hydrogen Sulfide Ameliorates Cognitive Dysfunction in Formaldehyde-Exposed Rats: Involvement in the Upregulation of Brain-Derived Neurotrophic Factor
| Autor: | Ming Xie, Xiao-Qing Tang, Lei Wu, Bo Wang, Yuan-Yuan Zhuang, Xiang Li, Hong-Feng Gu |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Morris water navigation task Sodium hydrosulfide Hippocampal formation Rats Sprague-Dawley Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neurotrophic factors Formaldehyde Internal medicine medicine Animals Learning Hippocampus (mythology) Cognitive Dysfunction Hydrogen Sulfide CA1 Region Hippocampal Biological Psychiatry Brain-derived neurotrophic factor TUNEL assay Behavior Animal biology Gasotransmitters Sulfates Chemistry Brain-Derived Neurotrophic Factor Rats Up-Regulation 030227 psychiatry Disease Models Animal Psychiatry and Mental health Neuropsychology and Physiological Psychology Endocrinology biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Neuropsychobiology. 79:119-130 |
| ISSN: | 1423-0224 0302-282X |
| DOI: | 10.1159/000501294 |
| Popis: | Objective: To investigate whether hydrogen sulfide (H2S) counteracts formaldehyde (FA)-induced cognitive defects and whether the underlying mechanism is involved in the upregulation of hippocampal brain-derived neurotrophic factor (BDNF) expression. Methods: The cognitive function of rats was evaluated by the Morris water maze (MWM) test and the novel object recognition test. The content of superoxide dismutase (SOD) and malondialdehyde (MDA) in the hippocampus were detected by enzyme-linked immunosorbent assay (ELISA). The neuronal apoptosis in the hippocampal CA1 region was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end (TUNEL) staining. The expression of the BDNF protein was detected by Western blot and immunohistochemistry. Results: We found that sodium hydrosulfide (NaHS, a donor of H2S) significantly reversed the impairment in the function of learning and memory in the MWM test and the novel objective recognition task induced by intracerebroventricular injection of FA. We also showed that NaHS significantly reduced the level of MDA, elevated the level of SOD, and decreased the amount of TUNEL-positive neurons in the hippocampus of FA-exposed rats. Moreover, NaHS markedly increased the expression of hippocampal BDNF in FA-exposed rats. Conclusions: H2S attenuates FA-induced dysfunction of cognition and the underlying mechanism is involved in the reduction of hippocampal oxidative damage and apoptosis as well as upregulation of hippocampal BDNF. |
| Databáze: | OpenAIRE |
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