Lanthanide-Coordinated Semiconducting Polymer Dots Used for Flow Cytometry and Mass Cytometry
| Autor: | I-Che Wu, Li Wu, Xu Wu, Fangmao Ye, William W. Kwok, Daniel T. Chiu, Jiangbo Yu, Chun-Ting Kuo, Quinn DeGottardi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lanthanide Polymers 02 engineering and technology 010402 general chemistry Semiconducting polymer Lanthanoid Series Elements 01 natural sciences Peripheral blood mononuclear cell Article Catalysis Flow cytometry Jurkat Cells 03 medical and health sciences Microscopy Electron Transmission medicine Humans Mass cytometry medicine.diagnostic_test Chemistry Disease mechanisms General Medicine General Chemistry 021001 nanoscience & nanotechnology Flow Cytometry Fluorescence Peripheral blood 0104 chemical sciences Spectrometry Fluorescence 030104 developmental biology Semiconductors Molecular Probes Leukocytes Mononuclear MCF-7 Cells Biophysics 0210 nano-technology Biomarkers |
| Zdroj: | Angewandte Chemie International Edition. 56:14908-14912 |
| ISSN: | 1433-7851 |
| Popis: | Simultaneous monitoring of biomarkers as well as single-cell analyses based on flow cytometry and mass cytometry are important for investigations of disease mechanisms, drug discovery, and signaling-network studies. Flow cytometry and mass cytometry are complementary to each other; however, probes that can satisfy all the requirements for these two advanced technologies are limited. In this study, we report a probe of lanthanide-coordinated semiconducting polymer dots (Pdots), which possess fluorescence and mass signals. We demonstrated the usage of this dual-functionality probe for both flow cytometry and mass cytometry in a mimetic cell mixture and human peripheral blood mononuclear cells as model systems. The probes not only offer high fluorescence signal for use in flow cytometry, but also show better performance in mass cytometry than the commercially available counterparts. |
| Databáze: | OpenAIRE |
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