Comparative analysis of the antibacterial and drug-modulatory effect of d-limonene alone and complexed with β-cyclodextrin

Autor: Fábia F. Campina, Adriano Antunes de Souza Araújo, Ana Raquel Pereira da Silva, Lucindo José Quintans-Júnior, Henrique Douglas Melo Coutinho, Raimundo Luiz Silva Pereira, Thiago Sampaio de Freitas, Alexandre Magno Rodrigues Teixeira, Janaína Esmeraldo Rocha, Irwin Rose Alencar de Menezes, Rafael Pereira da Cruz, Maria do Socorro Costa
Rok vydání: 2019
Předmět:
Zdroj: European Journal of Pharmaceutical Sciences. 128:158-161
ISSN: 0928-0987
DOI: 10.1016/j.ejps.2018.11.036
Popis: With the increase in bacterial resistance to antibiotics, many studies have been directed towards finding new agents with antibacterial activity, such as studies with natural products. These products can have antibacterial activity such as d -limonene as described in the literature. The aim of this study was to evaluate the antibacterial activity of d -limonene, isolated and complexed with β-cyclodextrin, and to evaluate its potentiating activity of different antibiotic classes. Antibacterial activity was determined by the broth microdilution method, obtaining in this way the Minimal Inhibitory Concentration (MIC), with the antibiotic modulatory activity being obtained using a sub-inhibitory concentration (MIC/8). d -Limonene showed a MIC equal to 256 μg/mL against standard S. aureus and 512 μg/mL against resistant P. aeruginosa. In the gentamicin modulatory activity, the isolated d -limonene presented synergism against S. aureus and E. coli bacteria. Thus, d -limonene showed relevant clinical antibacterial activity, for both Gram-positive and Gram-negative bacteria as well as a synergistic effect when associated with gentamicin. These results are promising in the combat against bacterial resistance, however further studies are needed to better elucidate the mechanisms of action.
Databáze: OpenAIRE
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