Safety Evaluation of Neo Transgenic Pigs by Studying Changes in Gut Microbiota Using High-Throughput Sequencing Technology

Autor: Maoxue Tang, Qingqing Wang, Pengfei Gao, Shengwang Jiang, Jie Liu, Dezun Ma, Ke Jiang, Wentao Cui, Jian Hou, Chunbo Cai, Hegang Li, Lili Qian
Rok vydání: 2015
Předmět:
0301 basic medicine
Swine
Kanamycin kinase
Agricultural Biotechnology
lcsh:Medicine
Artificial Gene Amplification and Extension
Gut flora
medicine.disease_cause
Polymerase Chain Reaction
Animals
Genetically Modified
Feces
Antibiotics
Lactobacillus
Medicine and Health Sciences
Transgenes
lcsh:Science
Mammals
Multidisciplinary
biology
Antimicrobials
Genetically Modified Organisms
High-Throughput Nucleotide Sequencing
Drugs
Agriculture
Neomycins
Neomycin
Transgenic Engineering
Vertebrates
Anatomy
Genetic Engineering
medicine.drug
Research Article
Biotechnology
Livestock
Gene Transfer
Horizontal
Firmicutes
Transgene
030106 microbiology
Research and Analysis Methods
Microbiology
03 medical and health sciences
Ileum
Microbial Control
Drug Resistance
Bacterial
Proteobacteria
medicine
Escherichia coli
Animals
Molecular Biology Techniques
Molecular Biology
Pharmacology
Kanamycin Kinase
Genetically Modified Animals
Bacteria
Bacteroidetes
lcsh:R
Gut Bacteria
Organisms
Biology and Life Sciences
biology.organism_classification
Gastrointestinal Microbiome
Gastrointestinal Tract
030104 developmental biology
lcsh:Q
Hafnia
Shigella
Digestive System
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 3, p e0150937 (2016)
ISSN: 1932-6203
Popis: The neo (neomycin phosphotransferase) gene is widely used as a selection marker in the production of genetically engineered animals and plants. Recent attention has been focused on safety concerns regarding neo transgene expression. In this study, neo transgenic and non-transgenic piglets were randomly assigned into Group A and Group B to evaluate effects of neo transgene by studying changes in gut microbiota using high-throughput sequencing. Group A pigs were fed a standard diet supplemented with antibiotic neomycin; Group B pigs were fed a standard diet. We examined horizontal transfer of exogenous neo gene using multiplex PCR; and investigated if the presence of secreted NPT II (neo expression product) in the intestine could lead to some protection against neomycin in transgenic pigs by monitoring different patterns of changes in gut microbiota in Group A animals. The unintended effects of neo transgene on gut microbiota were studied in Group B animals. Horizontal gene transfer was not detected in gut microbiota of any transgenic pigs. In Group A, a significant difference was observed between transgenic pigs and non-transgenic pigs in pattern of changes in Proteobacteria populations in fecal samples during and post neomycin feeding. In Group B, there were significant differences in the relative abundance of phyla Firmicutes, Bacteroidetes and Proteobacteria, and genera Lactobacillus and Escherichia-Shigella-Hafnia between transgenic pigs and non-transgenic pigs. We speculate that the secretion of NPT II from transgenic tissues/cells into gut microbiota results in the inhibition of neomycin activity and the different patterns of changes in bacterial populations. Furthermore, the neo gene also leads to unintended effects on gut microbiota in transgenic pigs that were fed with basic diet (not supplemented with neomycin). Thus, our data in this study caution that wide use of the neo transgene in genetically engineered animals should be carefully considered and fully assessed.
Databáze: OpenAIRE
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