Cell-Laden Biomimetically Mineralized Shark-Skin-Collagen-Based 3D Printed Hydrogels for the Engineering of Hard Tissues
| Autor: | Carmen G. Sotelo, Rui L. Reis, Ricardo I. Pérez-Martín, Rogério P. Pirraco, Tiago H. Silva, Catarina F. Marques, Gabriela S. Diogo |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2020 |
| Předmět: |
In situ
Mineralized tissues Coprecipitation Simulated body fluid 0206 medical engineering Cell Biomedical Engineering 02 engineering and technology law.invention Biomaterials Mice mineralized tissues applications law Spectroscopy Fourier Transform Infrared medicine Animals 3D bioprinting Science & Technology Mineralizated tissues applications Tissue Engineering Chemistry technology industry and agriculture Bioprinting Hydrogels 3D printing Mineralization (soil science) 021001 nanoscience & nanotechnology 020601 biomedical engineering 3. Good health medicine.anatomical_structure Chemical engineering In situ mineralization Printing Three-Dimensional Self-healing hydrogels Sharks Collagen 0210 nano-technology Marine biomaterials |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 2373-9878 |
| Popis: | 9 pages Mineralization processes based on coprecipitation methods have been applied as a promising alternative to the most commonly used methods of polymer–ceramic combination, direct mixing, and incubation in simulated body fluid (SBF) or modified SBF. In the present study, for the first time, the in situ mineralization (ideally hydroxyapatite formation) of blue shark (Prionace glauca (PG)) collagen to fabricate 3D printable cell-laden hydrogels is proposed. In the first part, several parameters for collagen mineralization were tested until optimization. The hydroxyapatite formation was confirmed by FT-IR, XRD, and TEM techniques. In the second part, stable bioinks combining the biomimetically mineralized collagen with alginate (AG) (1:1, 1:2, 1:3, and AG) solution were used for 3D printing of hydrogels. The addition of Ca2+ ions into the system did present a synergistic effect: by one side, the in situ mineralization of the collagen occurred, and at same time, they were also useful to ionically cross-link the blends with alginate, avoiding the addition of any cytotoxic chemical cross-linking agent. Mouse fibroblast cell line survival during and after printing was favored by the presence of PG collagen as exhibited by the biological performance of the hydrogels. Inspired in a concept of marine byproduct valorization, 3D bioprinting of in situ mineralized blue shark collagen is thus proposed as a promising approach, envisioning the engineering of mineralized tissues |
| Databáze: | OpenAIRE |
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