Enzymic synthesis of l-ascorbic acid from synthetic and biological d-glucurono-1,4-lactone conjugates
| Autor: | I. B. Chatterjee, C. Sen Gupta, C. Ray Chaudhuri, S. Dutta Gupta |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Chlorobutanol
Infrared Rays Stereochemistry Guinea Pigs Biophysics Glucuronates Ascorbic Acid Biochemistry Lactones chemistry.chemical_compound Oximes Benz(a)Anthracenes Animals Glycosides Molecular Biology Semicarbazone Cyanohydrin chemistry.chemical_classification Goats Imidazoles Substrate (chemistry) Cell Biology Ascorbic acid Oxime Rats Semicarbazides Metabolism Enzyme chemistry Spectrophotometry Barbiturates Microsomes Liver Microsome Rabbits Lactone |
| Zdroj: | Analytical Biochemistry. 38:46-55 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(70)90154-5 |
| Popis: | d -Glucofuranosiduronolactone conjugates, namely, semicarbazone, oxime, cyanohydrin, and thioacetal of d -glucurono-1,4-lactone, were converted to l -ascorbic acid by an enzyme system present in rat, goat, or rabbit liver microsomes. Guinea pig liver microsomes were ineffective. Neither methyl- d -glucofuranosiduronolactone nor any glucopyranosiduronic acid conjugates examined were converted into l -ascorbic acid. Methods of preparation and properties of d -glucurono-1,4-lactone semicarbazone and d -glucurono-1,4-lactone oxime have been described and a tentative mechanism of enzymic reduction of the conjugates into l -gulono-1,4-lactone has been suggested. A conjugate of d -glucurono-1,4-lactone and imidazole, which is a substrate for the microsomal enzyme leading to synthesis of l -ascorbic acid, has been identified in the urine of rat treated with chloretone, barbital, and 1,2-benzanthracene. It has been indicated that the enhanced synthesis of l -ascorbic acid after administration of various drugs and toxic chemical compounds is due to the induced formation of this endogenous substrate. |
| Databáze: | OpenAIRE |
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