Chronic exposure of human macrophages in vitro to morphine and methadone induces a putative tolerant/dependent state
| Autor: | Manuel Delgado-Vélez, Yarelis Robles, Eddy Ríos-Olivares, José W. Rodríguez, Irmarie Morales, Ana M. Lugo-Chinchilla, Nimaris Bruno, Fernando L. Renaud, Luis Lizardo, Miguel Correa |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Narcotics Drug Time Factors Adolescent Narcotic Antagonists Phagocytosis media_common.quotation_subject Immunology Pharmacology In vivo medicine Humans Immunology and Allergy Cells Cultured media_common Analysis of Variance Dose-Response Relationship Drug Morphine Naloxone business.industry Macrophages Cell Differentiation Drug Tolerance In vitro Substance Withdrawal Syndrome Dose–response relationship Neurology Female Neurology (clinical) Analysis of variance business Methadone medicine.drug |
| Zdroj: | Journal of Neuroimmunology. 196:94-100 |
| ISSN: | 0165-5728 |
| Popis: | We have shown previously that whereas acute exposure of cultured murine peritoneal macrophages inhibits phagocytosis, chronic exposure results in a putative tolerant/dependent state. We now report similar observations using human cultured monocyte-derived macrophages (hMDM) from a control population and from methadone patients. With hMDM, acute exposure to morphine and methadone inhibited phagocytosis in a dose-dependent manner. In contrast, chronic exposure resulted in eventual normalization of phagocytosis, indicating that a putative tolerant state to the opiates had developed. When opiates were withdrawn from chronically-exposed, tolerized hMDM, phagocytosis was once again depressed. The duration of withdrawal-induced depression lasted several hours, which is much longer than evidenced previously with murine macrophages. These data identify well with various in vivo studies on immune effects of opiate withdrawal; and, in so-doing, supplement ongoing speculation that opiate withdrawal is likely to have serious impact on host defenses of street heroin addicts. |
| Databáze: | OpenAIRE |
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