| Popis: |
PDF file - 177K, T47D cells were starved and then treated or not with MPA for 10 min, and processed for immunofluorescence and confocal microscopy using specific antibodies to detect pSer162 PRB and ER� (SP1, A) or pSer294 PR and ER� (M7047, B). Bar: 15�m. Co-localization between nuclear receptors was quantified using the quantitative statistical analysis for nuclear pixel overlap after 10 min of MPA treatment and was estimated using the Pearson's correlation coefficient. (C) Cellular fractionation of T47D cells was controlled by Western blot using anti-tubulin (cytoplasmatic marker, Ab4 from Thermo) or anti-Sp1 (nuclear marker, from Thermo) antibodies. Erk1/2 was used as a loading control. (D) T47D cells were starved and then treated with ICI during 3, 6, 12, 24 and 48 h. ER� (SP1) and PR (Ab7) expression was analyzed by Western blot. Erk1/2 was used as a loading control. |
