Enhanced proliferation and dopaminergic differentiation of ventral mesencephalic precursor cells by synergistic effect of FGF2 and reduced oxygen tension
| Autor: | Jan Bert Gramsbergen, Hans Rudolf Widmer, Pia Jensen, Jens Zimmer, Morten Meyer |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Fibroblast Growth Factor 8 Tyrosine 3-Monooxygenase Dopamine Immunoblotting chemistry.chemical_element Biology Oxygen Immunoenzyme Techniques 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neural Stem Cells Mesencephalon Internal medicine Precursor cell medicine Animals Cells Cultured Cell Proliferation 030304 developmental biology 0303 health sciences L-Lactate Dehydrogenase Tyrosine hydroxylase Dopaminergic Cell Differentiation Cell Biology Rats Oxygen tension Endocrinology chemistry Stem cell 030217 neurology & neurosurgery Bromodeoxyuridine medicine.drug |
| Zdroj: | Jensen, P, Gramsbergen, J-B, Zimmer, J, Widmer, H R & Meyer, M 2011, ' Enhanced proliferation and dopaminergic differentiation of ventral mesencephalic precursor cells by synergistic effect of FGF2 and reduced oxygen tension ', Experimental Cell Research, vol. 317, no. 12, pp. 1649-62 . https://doi.org/10.1016/j.yexcr.2011.04.012 |
| ISSN: | 0014-4827 |
| Popis: | Effective numerical expansion of dopaminergic precursors might overcome the limited availability of transplantable cells in replacement strategies for Parkinson's disease. Here we investigated the effect of fibroblast growth factor-2 (FGF2) and FGF8 on expansion and dopaminergic differentiation of rat embryonic ventral mesencephalic neuroblasts cultured at high (20%) and low (3%) oxygen tension. More cells incorporated bromodeoxyuridine in cultures expanded at low as compared to high oxygen tension, and after 6 days of differentiation there were significantly more neuronal cells in low than in high oxygen cultures. Low oxygen during FGF2-mediated expansion resulted also in a significant increase in tyrosine hydroxylase-immunoreactive (TH-ir) dopaminergic neurons as compared to high oxygen tension, but no corresponding effect was observed for dopamine release into the culture medium. However, switching FGF2-expanded cultures from low to high oxygen tension during the last two days of differentiation significantly enhanced dopamine release and intracellular dopamine levels as compared to all other treatment groups. In addition, the short-term exposure to high oxygen enhanced in situ assessed TH enzyme activity, which may explain the elevated dopamine levels. Our findings demonstrate that modulation of oxygen tension is a recognizable factor for in vitro expansion and dopaminergic differentiation of rat embryonic midbrain precursor cells. |
| Databáze: | OpenAIRE |
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