Proangiogenic Peptide Nanofiber Hydrogels for Wound Healing
| Autor: | Jin-mei He, Bin Chu, Xiao-Li Li, Lingling You, Wang Song, Lan-lan Liu, Changsheng Chen, Chaoxi Wu, Mei Tu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Wound Healing Chemistry Angiogenesis Regeneration (biology) 0206 medical engineering Nanofibers Biomedical Engineering Hydrogels Peptide 02 engineering and technology 021001 nanoscience & nanotechnology 020601 biomedical engineering Umbilical vein Cell biology Biomaterials Mice In vivo Self-healing hydrogels Human Umbilical Vein Endothelial Cells Animals Peptides 0210 nano-technology Wound healing Peptide sequence |
| Zdroj: | ACS Biomaterials Science & Engineering. 7:1100-1110 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.0c01264 |
| Popis: | Rapid vascularization is vital for dermal regeneration, nutrient and nutrition transfer, metabolic waste removal, and prevention of infection. This study reports on a series of proangiogenic peptides designed to undergo self-assembly and promote angiogenesis and hence skin regeneration. The proangiogenic peptides comprised an angiogenic peptide segment, GEETEVTVEGLEPG, and a β-sheet structural peptide sequence. These peptides dissolved easily in ultrapure water and rapidly self-assembled into hydrogels in a pH-dependent manner, creating three-dimensional fibril network structures and nanofibers as revealed by a scanning microscope and a transmission electron microscope. In vitro experiments showed that the peptide hydrogels favored adhesion and proliferation of mouse fibroblasts (L929) and human umbilical vein endothelial cells (HUVECs). In particular, many connected tubes were formed in the HUVECs after 8 h of culture on the peptide hydrogels. In vivo experiments demonstrated that new blood vessels grew into the proangiogenic peptide hydrogels within 2 weeks after subcutaneous implantation in mice. Moreover, the proangiogenic-combined hydrogels exhibited faster repair cycles and better healing of skin defects. Collectively, the results indicate that the proangiogenic peptide hydrogels are a promising therapeutic option for skin regeneration. |
| Databáze: | OpenAIRE |
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