NeuCode Labels for Relative Protein Quantification *
| Autor: | Joshua J. Coon, Matthew E. MacGilvray, Derek J. Bailey, Alexander S. Hebert, Christopher M. Rose, Michael S. Westphall, Audrey P. Gasch, Anna E. Merrill, Marwan El Masri, Joel Chandler Bradley, William Wakefield Wood |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Saccharomyces cerevisiae Proteins Proteome Lysine Mutant Quantitative proteomics Technological Innovation and Resources Biology Sodium Chloride Mass spectrometry Biochemistry Yeast Analytical Chemistry Stress Physiological Stable isotope labeling by amino acids in cell culture Molecular Biology |
| Popis: | We describe a synthesis strategy for the preparation of lysine isotopologues that differ in mass by as little as 6 mDa. We demonstrate that incorporation of these molecules into the proteomes of actively growing cells does not affect cellular proliferation, and we discuss how to use the embedded mass signatures (neutron encoding (NeuCode)) for multiplexed proteome quantification by means of high-resolution mass spectrometry. NeuCode SILAC amalgamates the quantitative accuracy of SILAC with the multiplexing of isobaric tags and, in doing so, offers up new opportunities for biological investigation. We applied NeuCode SILAC to examine the relationship between transcript and protein levels in yeast cells responding to environmental stress. Finally, we monitored the time-resolved responses of five signaling mutants in a single 18-plex experiment. |
| Databáze: | OpenAIRE |
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