Presence of the cannabinoid receptors, CB1 and CB2, in human omental and subcutaneous adipocytes
| Autor: | Jean-François Baron, Laurence Hoareau, Régis Roche, Yacine Haffaf, Sandrine Bes-Houtmann, Christine Laborde, Maya Cesari, Franck Festy, Marie-Paule Gonthier |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_specialty Cytoplasm Histology Cannabinoid receptor Subcutaneous Fat Adipose tissue Stimulation White adipose tissue Biology Receptor Cannabinoid CB2 chemistry.chemical_compound Receptor Cannabinoid CB1 Internal medicine Adipocyte medicine Cannabinoid receptor type 2 Adipocytes Cyclic AMP Humans Receptor Molecular Biology Cell Membrane Cell Biology Middle Aged Endocannabinoid system Immunohistochemistry Medical Laboratory Technology Endocrinology chemistry lipids (amino acids peptides and proteins) Female Omentum |
| Zdroj: | Histochemistry and cell biology. 126(2) |
| ISSN: | 0948-6143 |
| Popis: | To investigate the expression of the endocannabinoid 1 and 2 receptors by human adipocyte cells of omental and subcutaneous fat tissue, as well as to determine whether these receptors are functional. The expression of CB1 and CB2 receptors on human adipocytes was analyzed by western blotting, immunohistology and immunocytology. We also investigated intracytoplasmic cyclic AMP level modulation following CB1 and CB2 receptor stimulation by an enzymatic immuno assay. All mature adipocytes, from visceral (epiploon) and subcutaneous fat tissue, express CB1 and CB2 on their plasma membranes. We also demonstrate in this study that adipocyte precursors (pre-adipocytes) express CB1 and CB2 on their plasma membranes and that both receptors are functional. Activation of CB1 increases intracytoplasmic cyclic AMP whilst CB2 activation leads to a cyclic AMP decrease. Here we demonstrate, for the first time, that adipocytes of human adipose tissue (mature adipocytes and pre-adipocytes) express functional plasma membrane CB1 and CB2 receptors. Their physiological role on the adipose tissue is not known. However, their major involvement in the physiology of other tissues leads us to suppose that they could play a significant role in the homeostasis of the energy balance and/or in the regulation of adipose tissue inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink