Changes in the urinary extracellular vesicle proteome are associated with nephronophthisis-related ciliopathies
| Autor: | Marc R. Lilien, Kirsten Y. Renkema, Ernie M.H.F. Bongers, Thang V. Pham, Irene V. Bijnsdorp, Connie R. Jimenez, Jaco C. Knol, Rachel H. Giles, Marijn Stokman, Nine V A M Knoers, Tim Schelfhorst, Sander R. Piersma |
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| Přispěvatelé: | Urology, Medical oncology laboratory, AGEM - Re-generation and cancer of the digestive system, Amsterdam Neuroscience - Neurodegeneration, AII - Inflammatory diseases |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Proteomics 0301 basic medicine Adolescent Proteome Biophysics PRIMARY CILIA Renal ciliopathy Urine Biology Biochemistry Extracellular Vesicles 03 medical and health sciences All institutes and research themes of the Radboud University Medical Center Nephronophthisis MARKERS medicine Humans Biomarker discovery Child LABEL-FREE IDENTIFICATION 030102 biochemistry & molecular biology MUTATIONS KIDNEY-DISEASE Biomarker Extracellular vesicle Kidney Diseases Cystic medicine.disease Actin cytoskeleton BIOMARKER DISCOVERY Ciliopathies Cell biology Ciliopathy TARGET 030104 developmental biology Kidney Failure Chronic Female Rare cancers Radboud Institute for Health Sciences [Radboudumc 9] Extracellular matrix organization |
| Zdroj: | Journal of Proteomics, 192, 27-36 Journal of Proteomics, 192, pp. 27-36 Stokman, M F, Bijnsdorp, I V, Schelfhorst, T, Pham, T V, Piersma, S R, Knol, J C, Giles, R H, Bongers, E M H F, Knoers, N V A M, Lilien, M R, Jiménez, C R & Renkema, K Y 2019, ' Changes in the urinary extracellular vesicle proteome are associated with nephronophthisis-related ciliopathies ', Journal of Proteomics, vol. 192, pp. 27-36 . https://doi.org/10.1016/j.jprot.2018.07.008 Journal of proteomics, 192, 27-36. ELSEVIER SCIENCE BV Journal of Proteomics, 192, 27. Elsevier Journal of Proteomics, 192, 27-36. Elsevier |
| ISSN: | 1874-3919 |
| DOI: | 10.1016/j.jprot.2018.07.008 |
| Popis: | Nephronophthisis is one of the leading genetic causes of end-stage renal disease in childhood. Early diagnostics and prognostics for nephronophthisis are currently limited. We aimed to identify non-invasive protein biomarkers for nephronophthisis in urinary extracellular vesicles. Extracellular vesicles were isolated from urine of 12 patients with a nephronophthisis-related ciliopathy and 12 age- and gender-matched controls, followed by in-depth label-free LC-MS/MS proteomics analysis of gel fractionated extracellular vesicle proteins. Supervised cluster analysis of proteomic profiles separated patients from controls. We identified 156 differentially expressed proteins with fold change ≥4 in patients compared to controls (P |
| Databáze: | OpenAIRE |
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