Analysis of ACE inhibitors in pharmaceutical dosage forms by derivative UV spectroscopy and liquid chromatography (HPLC)
| Autor: | Vanni Cavrini, Vincenza Andrisano, D. Bonazzi, Roberto Gotti |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Clinical Biochemistry
Pharmaceutical Science Benazepril Angiotensin-Converting Enzyme Inhibitors Capsules High-performance liquid chromatography Dosage form Analytical Chemistry chemistry.chemical_compound Spectrophotometry Drug Discovery medicine Chromatography High Pressure Liquid Spectroscopy Chromatography medicine.diagnostic_test Lisinopril Reversed-phase chromatography Hydrogen-Ion Concentration chemistry Enalapril Maleate Calibration Spectrophotometry Ultraviolet Derivative (chemistry) Tablets medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 16:431-438 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/s0731-7085(97)00075-7 |
| Popis: | Derivative UV spectroscopy and high performance liquid chromatography (HPLC) were applied to the determination of angiotensin-converting enzyme (ACE) inhibitors in their pharmaceutical dosage forms. For spectrophotometric determinations, the more appropriate derivative order was selected for each drug: ramipril (third-order), benazepril (second-order), enalapril maleate (second-order), lisinopril (first- and second-order) and quinapril (first-order). Reverse phase HPLC procedures (ODS column) were developed able to provide a single, symmetric peak for each drug; mixtures A-B, where A is 20 mM sodium heptansulphonate (pH 2.5) and B is acetonitrile-THF (95:5 v/v), proved to be suitable mobile phases to obtain selective separations of the cited ACE inhibitors. At ambient temperature, a low pH value (2.5) was found to be critical to avoid peak splitting and band broadening. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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