Microorganisms Isolated from Stored Meat in India, with Potential Antimicrobial Activity against Food Pathogens
| Autor: | S. Thiruvengadam, Gokila Thangavel |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Salmonella Staphylococcus aureus Meat 030106 microbiology Pharmaceutical Science India medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Listeria monocytogenes Food Preservation RNA Ribosomal 16S medicine Escherichia coli Leuconostoc Food microbiology Animals Humans Food science biology 04 agricultural and veterinary sciences MRS agar Antimicrobial biology.organism_classification 040401 food science chemistry Listeria Food Microbiology Nutrient agar Biotechnology |
| Zdroj: | Current pharmaceutical biotechnology. 20(5) |
| ISSN: | 1873-4316 |
| Popis: | Background:World Health Organization has estimated that 1 in 10 people fall ill and 4, 20, 000 die every year from eating contaminated food. Food pathogens like Escherichia, Salmonella, Staphylococcus and Listeria pose a serious threat to human health.Objective:The objective was to isolate microbes from meat stored at refrigerated conditions and evaluate the antimicrobial activity of the cell-free supernatant against food pathogens.Methods:Chicken and Pork samples were procured and stored at refrigerated conditions (4-7ºC) for 2 weeks. The samples were plated on to Nutrient agar (NA) and De Man, Rogosa and Sharpe (MRS) agar for isolation of aerobic and lactic acid bacteria. Cell-free supernatants of the isolates were screened for antimicrobial activity against Escherichia coli, Salmonella typhimurium, Listeria monocytogenes and Staphylococcus aureus by microtiter plate assay. The 5 most - effective strains were screened for hemolytic activity and identified by 16s rRNA sequencing.Results:A total of 110 strains were isolated, out of which the top 5 most - effective strains were all from MRS agar. They showed 88-90% inhibition against E. coli and S. typhimurium, whereas 60 to 70 % against S. aureus and L. monocytogenes. These strains were found to be non - hemolytic and were identified as Leuconostoc spp. namely, L. mesenteroides subsp. mesenteroides J18, CP003101; L. mesenteroides LM2; L. mesenteroides ATCC 8293, CP000414; L. gelidum subsp. gasicomitatum LM G 18811 and L. mesenteroides; LM2, AY675249.Conclusion:Leuconostoc are known to be effective in controlling foodborne pathogens and therefore, these strains have the potential for application in food and human. |
| Databáze: | OpenAIRE |
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