Heterogeneous expression of plasma-membrane-localised OsOSCA1.4 complements osmotic sensing based on hyperosmolality and salt stress in Arabidopsis osca1 mutant
| Autor: | Yuanjun Zhai, Ping Gao, Yang Han, Wenqing Zhuo, Zhaohong Wen, Jin Liu, Chao Xi, Shengcheng Han, Yingdian Wang, Fang Wang, Heping Zhao, Youjun Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Osmosis Physiology Mutant Arabidopsis Endoplasmic Reticulum Salt Stress 03 medical and health sciences 0302 clinical medicine Cytosol Guard cell Humans Molecular Biology Ion channel Calcium signaling Plant Proteins biology Chemistry Endoplasmic reticulum Protoplasts HEK 293 cells Cell Membrane Osmolar Concentration Oryza Cell Biology Protoplast biology.organism_classification Plants Genetically Modified Cell biology 030104 developmental biology HEK293 Cells Phenotype Mutation Mesophyll Cells 030217 neurology & neurosurgery Subcellular Fractions |
| Zdroj: | Cell calcium. 91 |
| ISSN: | 1532-1991 |
| Popis: | In plants, both hyperosmolality and salt stress induce cytosolic calcium increases within seconds, referred to as the hyperosmolality-induced [Ca2+]cyt increases, OICIcyt, and salt stress-induced [Ca2+]cyt increases, SICIcyt. Previous studies have shown that Arabidopsis reduced hyperosmolality-induced [Ca2+]i increase 1 (OSCA1.1) encodes a hyperosmolality-gated calcium-permeable channel that mediates OICIcyt in guard cells and root cells. Multiple OSCA members exist in plants; for example, Oryza sativa has 11 OsOSCAs genes, indicating that OSCAs have diverse biological functions. Here, except for OsOSCA4.1, ten full-length OsOSCAs were separately subcloned, in which OsOSCA1.4 was exclusively localised to the plasma membrane and other nine OsOSCAs-eYFP co-localised with an endoplasmic reticulum marker in Arabidopsis mesophyll protoplasts. OsOSCA1.4 was further identified as a calcium-permeable ion channel that activates an inward current after receiving an osmotic signal exerted by hyperosmolality or salt stress, and mediates OICIcyt and SICIcyt in human embryonic kidney 293 (HEK293) cells. Moreover, overexpression of OsOSCA1.4 in Arabidopsis osca1 mutant complemented osmotic Ca2+ signalling, root growth, and stomatal movement in response to hyperosmolality and salt stress. These results will facilitate further study of OsOSCA-mediated calcium signalling and its distinct roles in rice growth and development. |
| Databáze: | OpenAIRE |
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