Sophocarpine exert protective effect against ox-LDL-induced endothelial damage via regulating NF-κB signaling pathway
| Autor: | Taizhu Bo, Chonghui Jiang, Xiaolong Zi, Xiaoping Liu, Dianxiang Tan, Libao Cui, Yufen Deng, Gang Lei, Hengrong Fang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
animal structures Down-Regulation Apoptosis Applied Microbiology and Biotechnology Biochemistry Cell Line Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Alkaloids 0302 clinical medicine Humans Cytotoxicity Molecular Biology Chemistry Cell adhesion molecule fungi Organic Chemistry NF-kappa B Endothelial Cells NF-κB General Medicine Cell biology Lipoproteins LDL Endothelial stem cell IκBα 030104 developmental biology Cytoprotection 030220 oncology & carcinogenesis DNA fragmentation Signal transduction Signal Transduction Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 84:2104-2112 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | Oxidized low-density lipoprotein (ox-LDL) was known to induce endothelial cell injury to the progression of atherosclerosis (AS). Sophocarpine (SPC), a compound of sophora alkaloids isolated from the plant Sophora alopecuroides, has been shown to exhibit various pharmacological activities. This study was designed to investigate the protective effect of SPC on ox-LDL-induced endothelial cells and explored its underlying mechanism. Our results show that SPC pre-incubation ameliorated ox-LDL-mediated HAECs cytotoxicity, DNA fragmentation, and apoptosis in a dose-dependent manner. Moreover, SPC significantly downregulated the mRNA or protein expression level of pro-inflammatory mediators (TGF-β, IL-6, IL-1β, TNF-α) and pro-inflammatory vascular adhesion molecules (VCAM-1, ICAM-1, and E-selectin). Mechanistically, SPC pre-treatment downregulated IκBα expression and inhibited translocation of NF-κB in ox-LDL-mediated HAECs, overexpression of NF-κB p65 counteracted the cytoprotective and anti-apoptotic effect of SPC, suggesting that its action is dependent on NF-κB signaling pathway. Collectively, SPC suppresses ox-LDL-induced HAECs injury by inhibiting the NF-κB signaling pathway. |
| Databáze: | OpenAIRE |
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