Detection of mRNA of Telomerase Protein in Benign Naevi and Melanomas Using RNAscope
| Autor: | Magnus Stougaard, Jeanette Bæhr Georgsen, Torben Steiniche, Pii B Baltzarsen, Patricia Switten Nielsen |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Telomerase Skin Neoplasms Histology Proliferation index In situ hybridization Biology telomerase Pathology and Forensic Medicine Breslow Thickness 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine melanoma Humans Telomerase reverse transcriptase RNA Messenger RNA Neoplasm Melanoma Nevus neoplasms RNAscope Aged Aged 80 and over Messenger RNA Middle Aged medicine.disease Neoplasm Proteins Medical Laboratory Technology enzymes and coenzymes (carbohydrates) 030104 developmental biology Cell culture 030220 oncology & carcinogenesis embryonic structures Cancer research Female in situ hybridization hTERT |
| Zdroj: | Baltzarsen, P B, Georgsen, J B, Nielsen, P S, Steiniche, T & Stougaard, M 2020, ' Detection of mRNA of Telomerase Protein in Benign Naevi and Melanomas Using RNAscope ', Applied Immunohistochemistry and Molecular Morphology, vol. 28, no. 1, pp. 36-41 . https://doi.org/10.1097/PAI.0000000000000690 |
| Popis: | Telomerase is reactivated in most cancers and is possibly an early driver event in melanoma. Our aim was to test a novel in situ hybridization technique, RNAscope, for the detection of human telomerase reverse transcriptase (hTERT) mRNA in archival formalin-fixed, paraffin-embedded (FFPE) tissue and to compare the mRNA expression of melanomas and benign naevi. Furthermore, we wanted to see if hTERT mRNA could be a diagnostic or prognostic marker of melanoma. In situ hybridization for the detection of hTERT mRNA was performed on FFPE tissue of 17 melanomas and 13 benign naevi. We found a significant difference in the expression of hTERT mRNA between melanomas and benign naevi (P |
| Databáze: | OpenAIRE |
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