Genetic engineering of microalgae for enhanced lipid production
| Autor: | Christian Südfeld, Sarah D'Adamo, Ruud A. Weusthuis, René H. Wijffels, Camilo F. Muñoz, Mihris Ibnu Saleem Naduthodi, Maria J. Barbosa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Bio Process Engineering
Overexpression Omics Bioengineering Biology Gene editing Applied Microbiology and Biotechnology Metabolic engineering Increased lipid chemistry.chemical_compound Genome editing Matematikk og Naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476 [VDP] Microalgae Food science Fatty acids Polyunsaturated fatty acids (PUFA) Fatty acid synthesis VLAG chemistry.chemical_classification Triacylglycerols (TAG) Fatty acid Lipid metabolism Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 [VDP] Lipids chemistry Metabolic Engineering Biofuel Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 [VDP] Biofuels Genetic engineering Fatty Acids Unsaturated Heterologous expression Biotechnology Polyunsaturated fatty acid |
| Zdroj: | Biotechnology Advances 52 (2021) Biotechnology Advances, 52 Biotechnology Advances |
| ISSN: | 0734-9750 |
| Popis: | Microalgae have the potential to become microbial cell factories for lipid production. Their ability to convert sunlight and CO2 into valuable lipid compounds has attracted interest from cosmetic, biofuel, food and feed industries. In order to make microalgae-derived products cost-effective and commercially competitive, enhanced growth rates and lipid productivities are needed, which require optimization of cultivation systems and strain improvement. Advances in genetic tool development and omics technologies have increased our understanding of lipid metabolism, which has opened up possibilities for targeted metabolic engineering. In this review we provide a comprehensive overview on the developments made to genetically engineer microalgal strains over the last 30 years. We focus on the strategies that lead to an increased lipid content and altered fatty acid profile. These include the genetic engineering of the fatty acid synthesis pathway, Kennedy pathway, polyunsaturated fatty acid and triacylglycerol metabolisms and fatty acid catabolism. Moreover, genetic engineering of specific transcription factors, NADPH generation and central carbon metabolism, which lead to increase of lipid accumulation are also reviewed. |
| Databáze: | OpenAIRE |
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