The presence of (+)-S-adenosyl-L-methionine in the rat brain and its lack of effect on phenylethanolamine N-methyltransferase activity
| Autor: | Corinne Beaudouin, Gisele Haurat, Bernard Renaud, Jean Alex Laffitte |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
S-Adenosylmethionine Magnetic Resonance Spectroscopy Stereochemistry Ultraviolet Rays Biochemistry High-performance liquid chromatography Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Animals Chromatography High Pressure Liquid chemistry.chemical_classification Phenylethanolamine N-methyltransferase activity Phenylethanolamine N-Methyltransferase Diastereomer Substrate (chemistry) Brain Stereoisomerism In vitro Phenylethanolamine N-methyltransferase Rats Phenylethanolamine Kinetics Enzyme chemistry |
| Zdroj: | Journal of neurochemistry. 61(3) |
| ISSN: | 0022-3042 |
| Popis: | (+)-S-Adenosyl-l-methionine [(+)-SAM] was isolated from rat brain and was quantified by HPLC followed by UV spectrophotometric measurements and by 1H-NMR. Its estimated ratio in brain is 3% of total SAM. Because of its commercial unavailability, (+)-SAM was also prepared from chemically synthesized SAM by separation of the two diastereoisomers on a preparative reverse-phase Nucleosil C8 column. The (+) diastereoisomer thus obtained was then assayed in vitro both as an inhibitor and a substrate of phenylethanolamine N-methyltransferase. Enzymatic activity was measured by HPLC analysis. It was shown that (+)-SAM has no effect on phenylethanolamine N-methyltransferase activity; therefore, it is unlikely that (+)-SAM plays any possible role in regulation of adrenaline synthesis in the brain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text