A rapid method for determining the tuberculocidal activity of liquid chemical germicides
| Autor: | Carl E. Cerniglia, Bruce D. Erickson, Warren L. Campbell |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Mycobacterium bovis
Bacteriological Techniques Chromatography biology Disinfectant General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology Orders of magnitude (mass) chemistry.chemical_compound chemistry Glutaral Luminescent Measurements Bioluminescence Test organism Glutaraldehyde Transformation Bacterial Luciferases Bacteria Disinfectants |
| Zdroj: | Current microbiology. 43(2) |
| ISSN: | 0343-8651 |
| Popis: | A rapid, quantitative method has been developed for determining the tuberculocidal activity of liquid chemical germicides. In this method, a test strain of Mycobacterium bovis that carries the firefly luciferase gene is exposed to a germicide, and the surviving bacteria are detected by bioluminescence. The tuberculocidal activities of five commercially available glutaraldehyde-based disinfectants were tested, and all reduced the number of surviving mycobacteria by greater than five orders of magnitude. In contrast, a phenol-based disinfectant with tuberculocidal claims gave less than one order of magnitude reduction of the test organism. With this method for determining tuberculocidal activity, results can be obtained in less than one day, compared with weeks or months for the standard tuberculocidal assays. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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