GIP-Dependent Expression of Hypothalamic Genes
| Autor: | Suresh Ambati, Della-Fera Ma, Yang-Ho Choi, Clifton A. Baile, Duan J, Hartzell Dl |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Cart endocrine system medicine.medical_specialty Physiology Hypothalamus Gene Expression Incretin Stimulation Gastric Inhibitory Polypeptide Receptors Gastrointestinal Hormone Rats Sprague-Dawley Mice Internal medicine Gene expression medicine Animals GABRD RNA Messenger Receptor biology Feeding Behavior General Medicine Rats Endocrinology biology.protein Energy Metabolism CREB1 Biomarkers hormones hormone substitutes and hormone antagonists |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1802-9973 0862-8408 |
| DOI: | 10.33549/physiolres.932151 |
| Popis: | GIP (glucose dependent insulinotrophic polypeptide), originally identified as an incretin peptide synthesized in the gut, has recently been identified, along with its receptors (GIPR), in the brain. Our objective was to investigate the role of GIP in hypothalamic gene expression of biomarkers linked to regulating energy balance and feeding behavior related neurocircuitry. Rats with lateral cerebroventricular cannulas were administered 10 μg GIP or 10 μl artificial cerebrospinal fluid (aCSF) daily for 4 days, after which whole hypothalami were collected. Real time Taqman™ RT-PCR was used to quantitatively compare the mRNA expression levels of a set of genes in the hypothalamus. Administration of GIP resulted in up-regulation of hypothalamic mRNA levels of AVP (46.9±4.5 %), CART (25.9±2.7 %), CREB1 (38.5±4.5 %), GABRD (67.1±11 %), JAK2 (22.1±3.6 %), MAPK1 (33.8±7.8 %), NPY (25.3±5.3 %), OXT (49.1±5.1 %), STAT3 (21.6±3.8 %), and TH (33.9±8.5 %). In a second experiment the same set of genes was evaluated in GIPR-/- and GIPR+/? mice to determine the effect of lack of GIP stimulation on gene expression. In GIPR-/- mice expressions of the following genes were down-regulated: AVP (27.1±7.5 %), CART (28.3±3.7 %), OXT (25.2±5.8 %), PTGES (23.9±4.5 %), and STAT3 (8.8±2.3 %). These results suggest that AVP, CART, OXT and STAT3 may be involved in energy balance-related hypothalamic circuits affected by GIP. |
| Databáze: | OpenAIRE |
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