Effect of NMDA on proliferation and apoptosis in hippocampal neural stem cells treated with MK‑801
| Autor: | Quan‑Rui Ma, Hui‑Hui Zhou, Yu Shao, Juan Liu, Yin‑Xiu Ding, Yu‑Qing He, Juan Ding, Yi‑Wei Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research N-group (finite group theory) Hippocampal formation Pharmacology hippocampal neural stem cells 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) dizocilpine medicine Chemistry Neurotoxicity Articles General Medicine Cell cycle medicine.disease Neural stem cell schizophrenia Dizocilpine 030104 developmental biology nervous system Apoptosis N-methyl-D-aspartate NMDA receptor 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2018.6346 |
| Popis: | The purpose of the present study was to investigate effects of N-methyl-D-aspartate (NMDA) on proliferation and apoptosis of hippocampal neural stem cells (NSCs) treated with dizocilpine (MK-801). Cultures of hippocampal NSCs were randomly divided into four groups consisting of an untreated control, cells treated with MK-801, NMDA and a combination of MK801 and NMDA (M+N). Proliferative and apoptotic responses for each of the experimental groups were determined by MTS and flow cytometry. The results revealed that MK-801 and NMDA exerted significant effects on hippocampal NSCs proliferation. Cell survival rates decreased in MK-801, NMDA and M+N treated groups compared with the control group. Cells survival rates in NMDA and M+N treated groups increased compared with the MK-801 treated group. MK-801 and NMDA were demonstrated to significantly affect apoptosis in hippocampal NSCs. Total and early stages of apoptosis in MK-801 and NMDA groups significantly increased compared with the control group. Total and early apoptosis of NSCs in the M+N group significantly decreased compared with MK-801 and NMDA groups. Late apoptosis of NSCs in MK-801 and NMDA groups significantly decreased compared with the control group. Late apoptosis of NSCs in the M+N group significantly increased compared with MK-801 and NMDA groups. The present study revealed that MK-801 inhibited proliferation and increased apoptosis in hippocampal NSCs. NMDA may reduce the neurotoxicity induced by MK-801, which may be associated with its activity towards NMDA receptors and may describe a novel therapeutic target for the treatment of schizophrenia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|