Cell surface lectins of human granulocytes: their expression is modulated by mononuclear cells and granulocyte/macrophage colony-stimulating factor
| Autor: | Michel Monsigny, Claudine Kieda, Catherine Grillon |
|---|---|
| Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), LEGOUPIL, Laëtitia, Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1990 |
| Předmět: |
Macrophage colony-stimulating factor
Receptor expression [SDV]Life Sciences [q-bio] T-Lymphocytes Cell Separation Granulocyte Biochemistry 03 medical and health sciences 0302 clinical medicine Reference Values Granulocyte macrophage colony-stimulating factor receptor medicine Macrophage Humans Receptor Cells Cultured 030304 developmental biology 0303 health sciences B-Lymphocytes Chemistry Antibodies Monoclonal Granulocyte-Macrophage Colony-Stimulating Factor Cell biology [SDV] Life Sciences [q-bio] modulation Kinetics medicine.anatomical_structure Granulocyte macrophage colony-stimulating factor 030220 oncology & carcinogenesis Receptors Mitogen endogenous lectins Leukocytes Mononuclear Tetradecanoylphorbol Acetate Granulocyte colony-stimulating factor receptor medicine.drug Granulocytes |
| Zdroj: | Glycobiology Glycobiology, Oxford University Press (OUP), 1990, 1 (1), pp.33-38. ⟨10.1093/glycob/1.1.33⟩ Glycobiology, 1990, 1 (1), pp.33-38. ⟨10.1093/glycob/1.1.33⟩ |
| ISSN: | 0959-6658 1460-2423 |
| Popis: | International audience; This paper presents the characterization of a sugar-specific receptor on the surface of human circulating poly-morphonuclear cells. With the help of fluorescent neo-glycoproteins and flow cytometry, a receptor was identified as being specific for α-L-rhamnosyl residues. The number of receptors was 55 000/cell and their affinity reached 2 × 108 I mol−1. This number changed as a function of the biological state of the cells. Indeed, receptor expression was modulated by the presence of other cells. T cells and B cells increased the number of receptors on the granulocyte surface. Expression of the α-L-rhamnose-specific lectin was dependent on lymphocyte derived soluble factor(s), which induce(s) growth and differentiation of polymorphonuclear phagocytes. Granulocyte/ macrophage colony-stimulating factor (GM-CSF) specifically produced a significant increase in the number of receptors for α-L-rhamnose (2–10-fold/cell). This modulation was independent of protein kinase C activators such as phorbol ester, which produced no effect on α-L-rhamnose receptor expression. These findings demonstrate that GM-CSF may stimulate post differentiation functions and properties of mature granulocytes. |
| Databáze: | OpenAIRE |
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