Determining Sonication Effect on E. coli in Liquid Egg, Egg Yolk and Albumen and Inspecting Structural Property Changes by Near-Infrared Spectra

Autor: Zsanett Bodor, Andrea Taczmanne Bruckner, Csaba Nemeth, Csilla Mohácsi-Farkas, István Kertész, Dávid Nagy, József Felföldi, Viktória Zsom-Muha
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Sensors
Volume 21
Issue 2
Sensors, Vol 21, Iss 398, p 398 (2021)
ISSN: 1424-8220
DOI: 10.3390/s21020398
Popis: In this study, liquid egg, albumen, and egg yolk were artificially inoculated with E. coli. Ultrasound equipment (20/40 kHz, 180/300 W
30/45/60 min) with a circulation cooling system was used to lower the colony forming units (CFU) of E. coli samples. Frequency, absorbed power, energy dose, and duration of sonication showed a significant impact on E. coli with 0.5 log CFU/mL in albumen, 0.7 log CFU/mL in yolk and 0.5 log CFU/mL decrease at 40 kHz and 6.9 W absorbed power level. Significant linear correlation (p <
0.001) was observed between the energy dose of sonication and the decrease of E. coli. The results showed that sonication can be a useful tool as a supplementary method to reduce the number of microorganism in egg products. With near-infrared (NIR) spectra analysis we were able to detect the structural changes of the egg samples, due to ultrasonic treatment. Principal component analysis (PCA) showed that sonication can alter C&ndash
H, C&ndash
N, &ndash
OH and N&ndash
H bonds in egg. The aquagrams showed that sonication can alter the properties of H2O structure in egg products. The observed data showed that the absorbance of free water (1412 nm), water molecules with one (1440 nm), two (1462 nm), three (1472 nm) and four (1488 nm) hydrogen bonds, water solvation shell (1452 nm) and strongly bonded water (1512 nm) of the egg samples have been changed during ultrasonic treatment.
Databáze: OpenAIRE
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