Retrospective determination of HIV-1 status by a PCR method on paraffin wax embedded sections
| Autor: | Tomas Slavik, H van Zyl, M Wolfaardt, I W Simson |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Pathology
medicine.medical_specialty Polymerase Chain Reaction Sensitivity and Specificity Virus Pathology and Forensic Medicine law.invention Serology Paraffin wax law Predictive Value of Tests HIV Seropositivity medicine Humans Lymph node Polymerase chain reaction Retrospective Studies Acquired Immunodeficiency Syndrome biology General Medicine Proteinase K medicine.anatomical_structure Predictive value of tests biology.protein HIV-1 Lymph Lymph Nodes Research Article |
| Zdroj: | Journal of clinical pathology. 48(8) |
| ISSN: | 0021-9746 |
| Popis: | AIM--To develop a simple but reliable polymerase chain reaction (PCR) method to determine the HIV-1 status of patients on formalin fixed, paraffin wax embedded lymph node tissue. METHODS--Fifty lymph node specimens, 20 from HIV-1 seropositive and 30 from HIV-1 seronegative patients, were analysed. Lymph nodes with a variety of disease conditions were included in the study. Tissue sections were treated with a DNA extraction buffer containing proteinase K and the crude cell lysate was used in PCR analysis. Nested primers were used to amplify HIV-1 DNA sequences coding for gag, pol and env proteins. PCR products were demonstrated by polyacrylamide gel electrophoresis. Results were then compared with HIV-1 serology of the patients from whom the tissue was obtained. RESULTS--The PCR method yielded a specificity of 100%, a sensitivity of 95%, a positive predictive value of 100%, and a negative predictive value of 97% when compared with HIV-1 serology. The kappa statistic (0.958) showed an excellent agreement between the PCR method and serology. Furthermore, HIV-1 DNA was demonstrated in lymph node tissue from a serologically unconfirmed acquired immunodeficiency syndrome case necropsied in 1982. CONCLUSION--This PCR method is a simple and reliable means of retrospectively determining the HIV-1 status of patients using formalin fixed, paraffin wax embedded lymph node tissue. |
| Databáze: | OpenAIRE |
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