| Autor: |
Albert Y. Sun, Grace Y. Sun, Jerzyk W. Lazarewicz, Victor T. S. Leu |
| Rok vydání: |
1983 |
| Předmět: |
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| Zdroj: |
Neurochemistry International. 5:471-478 |
| ISSN: |
0197-0186 |
| DOI: |
10.1016/0197-0186(83)90077-3 |
| Popis: |
Rat brain synaptosomes prelabeled with [ 14 C]arachidonate in their phospholipids were superfused with well oxygenated Krebs-Ringer-bicarbonate solution containing 0.2% BSA and subsequently depolarized by elevating the K + concentration in the superfusion medium from 5 to 55 mM. The efflux of labeled arachidonate at steady state was 0.19% ( n = 12) of total radioactivity per min. In the presence of 2.5 mM Ca 2+ , high K + (55 mM) in the medium elicited an increase in arachidonate efflux which amounted to 121.4% ( n = 6) of control. Both Ca 2+ and BSA were required for the stimulated efflux of arachidonate during K + -depolarization. Under the same condition, K + -stimulation also evoked the release of [ 3 H]norepinephrine which was preloaded into the synaptosomes prior to superfusion. EGTA abolished the stimulated release of both arachidonate and norepinephrine during K + -depolarization. These results, together with the loss of labeled arachidonic acid from phospholipids (Majewska and Sun, 1982), indicate that deacylation of membrane lipids is involved in synaptic functions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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