Expression Analysis of the 3 G-Protein Subunits, Gα, Gβ, and Gγ, in the Olfactory Receptor Organs of Adult Drosophila melanogaster
| Autor: | Carolina Gomez-Diaz, Esther Alcorta, Tamara Boto |
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| Rok vydání: | 2010 |
| Předmět: |
Olfactory system
Physiology G protein Gene Expression Olfaction Biology Olfactory Receptor Neurons Behavioral Neuroscience GTP-Binding Protein gamma Subunits Physiology (medical) Heterotrimeric G protein medicine Animals Olfactory receptor GTP-Binding Protein beta Subunits Anatomy biology.organism_classification Immunohistochemistry GTP-Binding Protein alpha Subunits Sensory Systems Cell biology Drosophila melanogaster medicine.anatomical_structure Signal transduction Transduction (physiology) |
| Zdroj: | Chemical Senses. 35:183-193 |
| ISSN: | 1464-3553 0379-864X |
| DOI: | 10.1093/chemse/bjp095 |
| Popis: | In many species, olfactory transduction is triggered by odorant molecules that interact with olfactory receptors coupled to heterotrimeric G-proteins. The role of G-protein-linked transduction in the olfaction of Drosophila is currently under study. Here, we supply a thorough description of the expression in the olfactory receptor organs (antennae and maxillary palps) of all known Drosophila melanogaster genes that encode for G-proteins. Using RT-polymerase chain reaction, we analyzed 6 Galpha (G(s), G(i), G(q), G(o), G(f), and concertina), 3 Gbeta (G(beta5), G(beta13F), and G(beta76C)), and 2 Ggamma genes (G(gamma1) and G(gamma30A)). We found that all Galpha protein-encoding genes showed expression in both olfactory organs, but G(f) mRNA was not detected in palps. Moreover, all the Gbeta and Ggamma genes are expressed in antennae and palps, except for G(beta76C). To gain insight into the hypothesis of different G-protein subunits mediating differential signaling in olfactory receptor neurons (ORNs), we performed immunohistochemical studies to observe the expression of several Galpha and Gbeta proteins. We found that Gs, Gi, Gq, and G(beta13F) subunits displayed generalized expression in the antennal tissue, including ORNs support cells and glial cells. Finally, complete coexpression was found between Gi and Gq, which are mediators of the cyclic adenosine monophosphate and IP3 transduction cascades, respectively. |
| Databáze: | OpenAIRE |
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