Mechanism of Resistance to Enteroviruses of Some Primate Cells in Tissue Culture
| Autor: | T. I. Krispin, V. D. Soloviev, Vadim I. Agol, V. G. Zaslavsky |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
viruses
Immunology Coxsackievirus Infections Coxsackievirus Virus Replication medicine.disease_cause Microbiology Virus Tissue culture Culture Techniques Virology Animal Viruses medicine Animals Humans Enterovirus biology Poliovirus Temperature RNA Haplorhini biology.organism_classification Viral replication Cell culture Insect Science Hybridization Genetic Adsorption Poliomyelitis |
| Zdroj: | Journal of Virology. 2:553-557 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.2.6.553-557.1968 |
| Popis: | Two cell lines, M10-45-2 and L-41, were studied, each of which possessed specific resistance either to poliovirus or to coxsackievirus. Infection of M10-45-2 cells with poliovirus ribonucleic acid (RNA) and L-41 cells with infectious coxsackievirus RNA was accompanied by production of complete viruses in each of the resistant cell lines. During incubation of the cells with the virus to which they were resistant, the amount of infectious virus did not decrease. Treatment with glycine-HCl buffer solution ( p H 2.5) of resistant M10-45-2 cells after incubation with poliovirus at 0 C did not result in recovery of infectious virus, although such release did take place after treatment of sensitive M10 cells. Infection of resistant cells with virus containing poliovirus RNA and coxsackievirus proteins resulted in production of poliovirus in M10-45-2 cells but not in L-41 cells. The resistant cells are apparently unable to adsorb the virus to which they are resistant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|