Analysis of xyloglucans by ambient chloride attachment ionization tandem mass spectrometry
| Autor: | Hilkka I. Kenttämaa, Vanessa A. Gallardo, Nicholas C. Carpita, John F. Klimek, Nelson R. Vinueza |
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| Rok vydání: | 2013 |
| Předmět: |
Polymers and Plastics
Electrospray ionization Arabidopsis Tandem mass spectrometry Chloride Oligomer Dissociation (chemistry) Polymerization Adduct Xyloglucans chemistry.chemical_compound Chlorides Tandem Mass Spectrometry medicine Materials Chemistry Sequencing Derivatization Glucans Chromatography Chemistry Organic Chemistry Galactosyltransferases Xyloglucan Hymenaea courbaril Arabidopsis thaliana Mutation mur3 Chloride attachment Xylans Hymenaea medicine.drug |
| Zdroj: | Carbohydrate Polymers. 98(1):1203-1213 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2013.06.070 |
| Popis: | Xyloglucan oligomers obtained upon enzyme digestion from Hymenaea courbaril, Arabidopsis Columbia-0 and mur3 were ionized and analyzed by using chloride anion attachment electrospray ionization (ESI) and tandem mass spectrometry. MW determination and structural elucidation of several xyloglucan oligomers was performed directly from the mixture solutions without sample pretreatment or derivatization. Sodium cation attachment was used to determine the number of xyloglucans present in the mixtures and their MWs. However, tandem mass spectrometry results showed that structure elucidation based on the sodium adducts is ambiguous. Chloride anion also forms stable adducts with these xyloglucans upon ESI. These adducts can be readily identified due to the chlorine isotope pattern. The mass spectral profile of xyloglucans obtained for the mixtures matches the HPAEC results, thus validating this methodology for the determination of the xyloglucan composition and the MW of each xyloglucan. Upon collisional activation in MS2 experiments, the chloride anion adducts readily lose HCl, which helps verify the molecular weight of each xyloglucan. Isolating the resulting anion (deprotonated oligomer) and subjecting it to further collision-activated dissociation experiments (MSn; n=3–4) yields useful structural information that allows the differentiation between isomeric anions and hence determination of the sequence of the xyloglucan oligomers. The deprotonated oligomers fragment by a stepwise loss of sugar units from the reducing end. |
| Databáze: | OpenAIRE |
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