Comparative genomic and functional analysis of Lactobacillus casei and Lactobacillus rhamnosus strains marketed as probiotics
| Autor: | Justus Reunanen, Cinzia Lucia Randazzo, Ingemar von Ossowski, Airi Palva, Seppo Salminen, Taija E. Pietilä, Pia Laine, Reetta Satokari, Hanna M. Järvinen, Cinzia Caggia, François P. Douillard, Willem M. de Vos, Ravi Kant, Angela Ribbera, Lars Paulin |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Gene clusters
Applied Microbiology and Biotechnology Genome Pilus law.invention Comparative genomic Genomic stability Lactobacillus casei Lactobacillus rhamnosus strain Metabolic capacities Probiotic stress fluids and secretions law Microbiologie Lactobacillus Genotype Gene cluster 2. Zero hunger Genetics lactic-acid bacteria 0303 health sciences Ecology biology Lacticaseibacillus rhamnosus food and beverages gg Bacterial Typing Techniques Lacticaseibacillus casei adhesion Phenotype binding-protein Biotechnology in-vitro Microbiology 03 medical and health sciences Lactobacillus rhamnosus bl23 mucus 030304 developmental biology VLAG 030306 microbiology Probiotics Genetic Variation sequence biology.organism_classification carbohydrate-metabolism Food Microbiology Food Science |
| Zdroj: | Applied and Environmental Microbiology, 79(6), 1923-1933 Applied and Environmental Microbiology 79 (2013) 6 |
| ISSN: | 0099-2240 |
| Popis: | Four Lactobacillus strains were isolated from marketed probiotic products, including L. rhamnosus strains from Vifit (Friesland Campina) and Idoform (Ferrosan) and L. casei strains from Actimel (Danone) and Yakult (Yakult Honsa Co.). Their genomes and phenotypes were characterized and compared in detail with L. casei strain BL23 and L. rhamnosus strain GG. Phenotypic analysis of the new isolates indicated differences in carbohydrate utilization between L. casei and L. rhamnosus strains, which could be linked to their genotypes. The two isolated L. rhamnosus strains had genomes that were virtually identical to that of L. rhamnosus GG, testifying to their genomic stability and integrity in food products. The L. casei strains showed much greater genomic heterogeneity. Remarkably, all strains contained an intact spaCBA pilus gene cluster. However, only the L. rhamnosus strains produced mucus-binding SpaCBA pili under the conditions tested. Transcription initiation mapping demonstrated that the insertion of an iso -IS 30 element upstream of the pilus gene cluster in L. rhamnosus strains but absent in L. casei strains had constituted a functional promoter driving pilus gene expression. All L. rhamnosus strains triggered an NF-κB response via Toll-like receptor 2 (TLR2) in a reporter cell line, whereas the L. casei strains did not or did so to a much lesser extent. This study demonstrates that the two L. rhamnosus strains isolated from probiotic products are virtually identical to L. rhamnosus GG and further highlights the differences between these and L. casei strains widely marketed as probiotics, in terms of genome content, mucus-binding and metabolic capacities, and host signaling capabilities. |
| Databáze: | OpenAIRE |
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