| Autor: |
Wakana Saso, Masako Yamasaki, Shin-ichi Nakakita, Shuetsu Fukushi, Kana Tsuchimoto, Noriyuki Watanabe, Nongluk Sriwilaijaroen, Osamu Kanie, Masamichi Muramatsu, Yoshimasa Takahashi, Tetsuro Matano, Makoto Takeda, Yasuo Suzuki, Koichi Watashi |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
PLoS pathogens. 18(6) |
| ISSN: |
1553-7374 |
| Popis: |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been transmitted across all over the world, in contrast to the limited epidemic of genetically- and virologically-related SARS-CoV. However, the molecular basis explaining the difference in the virological characteristics among SARS-CoV-2 and SARS-CoV has been poorly defined. Here we identified that host sialoglycans play a significant role in the efficient spread of SARS-CoV-2 infection, while this was not the case with SARS-CoV. SARS-CoV-2 infection was significantly inhibited by α2-6-linked sialic acid-containing compounds, but not by α2–3 analog, in VeroE6/TMPRSS2 cells. The α2-6-linked compound bound to SARS-CoV-2 spike S1 subunit to competitively inhibit SARS-CoV-2 attachment to cells. Enzymatic removal of cell surface sialic acids impaired the interaction between SARS-CoV-2 spike and angiotensin-converting enzyme 2 (ACE2), and suppressed the efficient spread of SARS-CoV-2 infection over time, in contrast to its least effect on SARS-CoV spread. Our study provides a novel molecular basis of SARS-CoV-2 infection which illustrates the distinctive characteristics from SARS-CoV. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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