CGplus, a standardized herbal composition ameliorates non-alcoholic steatohepatitis in a tunicamycin-induced mouse model
| Autor: | Chang-Kyu Byun, Myong-Min Lee, Hyeong-Geug Kim, Samkeun Lee, Won-Yong Kim, Jin-Seok Lee, Chang-Gue Son, Seung-Hoon Choi, Pung-Mi Hyun, Sung-Bae Lee |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Pharmaceutical Science Aspartate transaminase Pharmacology Protective Agents Salvia miltiorrhiza Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Non-alcoholic Fatty Liver Disease Drug Discovery medicine Animals Aspartate Aminotransferases Triglycerides biology business.industry Tumor Necrosis Factor-alpha Tunicamycin Fatty liver Alanine Transaminase medicine.disease Lipid Metabolism Mice Inbred C57BL Disease Models Animal 030104 developmental biology Complementary and alternative medicine Alanine transaminase chemistry Liver biology.protein Molecular Medicine Cytokines Lipid Peroxidation Steatosis Steatohepatitis business Drugs Chinese Herbal |
| Zdroj: | Phytomedicine : international journal of phytotherapy and phytopharmacology. 41 |
| ISSN: | 1618-095X |
| Popis: | Background The prevalence of Non-alcoholic fatty liver disease (NAFLD) including non-alcoholic steatohepatitis (NASH) has increased by 15–39% worldwide, but no pharmaceutical therapeutics exists. Hypothesis/Purpose This study investigated anti-hepatosteatotic effect of CGplus (a standardized herbal composition of Artemisia iwayomogi, Amomum xanthioides, and Salvia miltiorrhiza) and its underlying mechanisms in a tunicamycin-induced NASH model. Methods C57/BL6J male mice were orally administrated CGplus (50, 100, or 200 mg/kg), dimethyl dimethoxy biphenyl dicarboxylate (DDB, 50 mg/kg) or distilled water daily for 5 days. 18 h after a single injection of tunicamycin (ip, 2 mg/kg), the parameters for hepatic steatosis and inflammation were measured. Results Pretreatment with CGplus significantly attenuated the accumulation of triglycerides and total cholesterol as well as lipid peroxidation, evidenced by quantitative and histopathological analyses in liver tissues. The elevations of serum aspartate transaminase, alanine transaminase and lactate dehydrogenase were significantly ameliorated by CGplus. Also, it normalized the altered activities of pro- (TNF-α, IL-1β and IL-6), anti-inflammatory (IL-10) cytokines and lipid metabolism-related molecules in protein and gene expression analyses. Conclusion Our data present experimental evidence for the potential of CGplus as an herbal therapeutic against NAFLD and NASH. Its underlying mechanisms may involve the modulations of pro- and anti-inflammatory cytokines, but further study is required especially for the actions of CGplus on lipid metabolisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect