The genomics of ecological flexibility, large brains,and long lives in capuchin monkeys revealedwith fecalFACS

Autor: Carmen Soto Valverde, Jason A. Hodgson, Joseph D. Orkin, Mareike C. Janiak, Wesley C. Warren, Roman Krawetz, Daniela Tejada-Martinez, Lukas F. K. Kuderna, Anthony Di Fiore, Maria Pia Martin, Esther Lizano, Yoshihito Niimura, Claudia Fontsere, Shoji Kawamura, George H. Perry, Michael J. Montague, Saul Cheves Hernandez, João Pedro de Magalhães, Jia Tang, Tomas Marques-Bonet, Amanda D. Melin, Marc de Manuel, Javier del Campo
Přispěvatelé: Washington University in St. Louis, Canada Research Chairs, Natural Sciences and Engineering Research Council of Canada, Alberta Children's Hospital Research Institute, Generalitat de Catalunya, Japan Society for the Promotion of Science, Methuselah Foundation, Comisión Nacional de Investigación Científica y Tecnológica (Chile), Biotechnology and Biological Sciences Research Council (UK), European Research Council, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Howard Hughes Medical Institute, Fundación 'la Caixa', Natural Environment Research Council (UK)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Digital.CSIC. Repositorio Institucional del CSIC
instname
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
Popis: Ecological flexibility, extended lifespans, and large brains have long intrigued evolutionary biologists, and comparative genomics offers an efficient and effective tool for generating new insights into the evolution of such traits. Studies of capuchin monkeys are particularly well situated to shed light on the selective pressures and genetic underpinnings of local adaptation to diverse habitats, longevity, and brain development. Distributed widely across Central and South America, they are inventive and extractive foragers, known for their sensorimotor intelligence. Capuchins have among the largest relative brain size of any monkey and a lifespan that exceeds 50 y, despite their small (3 to 5 kg) body size. We assemble and annotate a de novo reference genome for Cebus imitator. Through high-depth sequencing of DNA derived from blood, various tissues, and feces via fluorescence-activated cell sorting (fecalFACS) to isolate monkey epithelial cells, we compared genomes of capuchin populations from tropical dry forests and lowland rainforests and identified population divergence in genes involved in water balance, kidney function, and metabolism. Through a comparative genomics approach spanning a wide diversity of mammals, we identified genes under positive selection associated with longevity and brain development. Additionally, we provide a technological advancement in the use of noninvasive genomics for studies of free-ranging mammals. Our intra- and interspecific comparative study of capuchin genomics provides insights into processes underlying local adaptation to diverse and physiologically challenging environments, as well as the molecular basis of brain evolution and longevity.
Funding was provided by Washington University in St. Louis, the Canada Research Chairs Program, and a National Sciences and Engineering Research Council of Canada Discovery Grant (to A.D.M.); the Alberta Children’s Hospital Research Institute (A.D.M., J.D.O., and M.C.J.); the Beatriu de Pinós postdoctoral programme of the Government of Catalonia’s Secretariat for Universities and Research of the Ministry of Economy and Knowledge 2017 BP 00265 (to J.D.O.); and the Japan Society for the Promotion of Science 15H02421 and 18H04005 (to S.K.). This work was partly funded by a Methuselah Foundation grant (to J.P.d.M.); and the Comisión Nacional de Investigación Científica y Tecnológica Chile through the doctoral studentship number 21170433 and the scholarship from the Higher Education Quality Improvement Program (MECESUP) AUS 2003 (to D.T.-M.). GenAge is funded by a Biotechnology and Biological Sciences Research Council Grant BB/R014949/1 (to J.P.d.M.). T.M.-B. is supported by funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 864203), BFU2017-86471-P (MINECO/FEDER, UE), “Unidad de Excelencia María de Maeztu”, funded by the Agencia Estatal de Investigación (CEX2018-000792-M), Howard Hughes International Early Career, Obra Social “La Caixa” and Secretaria d’Universitats i Recerca and CERCA Programme del Departament d’Economia i Coneixement de la Generalitat de Catalunya (GRC 2017 SGR 880). C.F. is supported by the “La Caixa” doctoral fellowship program. E.L. is supported by CGL2017-82654-P (MINECO/FEDER, UE). M.C.J. is supported by funding from the Natural Environment Research Council (NE/T000341/1).
With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2018-000792-M).
Databáze: OpenAIRE
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