Hsa_circ_0001021 regulates intestinal epithelial barrier function via sponging miR-224-5p in ulcerative colitis
| Autor: | Bing Li, Chang Liu, Yanbo Yu, Lixiang Li, Yan Li, Xiang Gu, Yu Yu, Xin Long, Xiu-Li Zuo |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Cancer Research Colon Cell Down-Regulation Biology Occludin Downregulation and upregulation Genetics medicine Humans In patient Intestinal Mucosa Smad4 Protein Epithelial Cells RNA Circular Middle Aged medicine.disease Ulcerative colitis Up-Regulation MicroRNAs Colonic mucosa medicine.anatomical_structure Zonula Occludens-1 Protein Cancer research Epithelial barrier function Colitis Ulcerative Female |
| Zdroj: | Epigenomics. 13:1385-1401 |
| ISSN: | 1750-192X 1750-1911 |
| Popis: | Aims: Few circRNAs have been thoroughly explored in ulcerative colitis (UC). Materials & methods: Microarrays and qualitative real-time PCRs were used to detect and confirm dysregulated circRNAs associated with UC. Functional analysis was performed to explore the roles. Results: A total of 580 circRNAs and 87 miRNAs were simultaneously dysregulated in both inflamed and noninflamed UC colonic mucosa compared with healthy controls. Accordingly, hsa_circ_0001021 was significantly downregulated in patients with UC and was related to Mayo scores. Clinical samples and cell experiments revealed that hsa_circ_0001021 was expressed in epithelial cells and correlated with ZO-1, occludin and CLDN-2. Moreover, hsa_circ_0001021 sponged miR-224-5p to upregulate smad4 and increased ZO-1 and occludin. Conclusion: Hsa_circ_0001021 is related to UC severity and regulates epithelial barrier function via sponging miR-224-5p. |
| Databáze: | OpenAIRE |
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