Differential use of immunoglobulin light chain genes and B lymphocyte expansion at sites of disease in rheumatoid arthritis (RA) compared with circulating B lymphocytes

Autor: Ravinder N. Maini, S P Moyes, Rizgar A. Mageed
Rok vydání: 1998
Předmět:
Male
Lymphocyte
Immunology
Molecular Sequence Data
Immunoglobulin Variable Region
Immunoglobulin light chain
Immunoglobulin E
Immunoglobulin alpha-Chains
Lymphocyte Activation
Arthritis
Rheumatoid
Immunoglobulin kappa-Chains
Immune system
Antigen
Immunoglobulin lambda-Chains
Sequence Homology
Nucleic Acid
medicine
Immunology and Allergy
Gene Rearrangement
B-Lymphocyte
Light Chain
Humans
Amino Acid Sequence
Aged
Autoimmune disease
Aged
80 and over
B-Lymphocytes
biology
Base Sequence
Genes
Immunoglobulin
Sequence Homology
Amino Acid
business.industry
Middle Aged
medicine.disease
Complementarity Determining Regions
medicine.anatomical_structure
Blood Circulation
biology.protein
Immunoglobulin Joining Region
Female
Immunoglobulin Light Chains
Original Article
Synovial membrane
Antibody
business
Zdroj: Clinical and experimental immunology. 113(2)
ISSN: 0009-9104
Popis: SUMMARYThe presence of germinal centre-like structures and clonotypic expansion of lymphocytes in RA synovia may indicate a site-specific immune response to local antigens, rather than passively entrapped immune cells, that sustains synovial inflammation. In this study we compare the nature of immunoglobulin light chain variable region gene use in the synovium of RA patients with peripheral B cells to determine the nature of the synovial immune response. Using Vλ and Vκ gene fingerprinting, which relies on differences in CDR3 length, we demonstrate differences in the pattern of Vλ and Vκ use and clonotypic expansion of B cells between the synovium and peripheral blood of RA patients. Further, we show that some synovial rearrangements with long CDR3 are selectively expanded. These longer than usual CDR3 were generated by a number of mechanisms including N-additions. However, the observed differences were not uniform in different patients. These observations suggest that local synovial antigens drive significant numbers of T and B lymphocytes selected from an existing repertoire shaped by genetic and environmental factors. Further, the data argue against passive retention of most B cells in the synovium of RA patients.
Databáze: OpenAIRE
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