Propulsive appliance stimulates the synthesis of insulin-like growth factors I and II in the mandibular condylar cartilage of young rats
| Autor: | Edna Teruko Kimura, Denise Hajjar, Marinilce Fagundes dos Santos |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cartilage
Articular Male medicine.medical_specialty medicine.medical_treatment Gene Expression In situ hybridization Mechanotransduction Cellular Condyle Dental Occlusion Immunoenzyme Techniques Insulin-like growth factor Insulin-Like Growth Factor II Proliferating Cell Nuclear Antigen Internal medicine medicine Animals RNA Messenger Insulin-Like Growth Factor I Rats Wistar General Dentistry Messenger RNA biology Insulin Cartilage Mandibular Condyle Cell Biology General Medicine Anatomy Rats Proliferating cell nuclear antigen Endocrinology medicine.anatomical_structure Otorhinolaryngology biology.protein Orthodontic Appliances Functional Immunohistochemistry Stress Mechanical |
| Zdroj: | Archives of Oral Biology. 48:635-642 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/s0003-9969(03)00128-6 |
| Popis: | Functional orthopedic appliances correct dental malocclusion partially by exerting indirect mechanical stimulus on the condylar cartilage, modulating growth and the adaptation of orofacial structures. However, the exact nature of the biological responses to this therapy is not well understood. Insulin-like growth factors I and II (IGF-I and IGF-II) are important local factors during growth and differentiation of several tissues, including cartilage. The aim of this study was to verify the mRNA and protein expression of IGF-I and IGF-II in the condylar cartilage of young male Wistar rats that used a mandibular propulsive appliance for 3, 5, 7, 9, 11, 13 or 15 days. For this purpose, sagittal sections of decalcified and paraffin-embedded condyles were submitted to immunohistochemistry and in situ hybridization. IGF-I and IGF-II expression increased with developmental age in the control and treated rats. After 9 days of treatment the positivity for both peptides in the animals that wore the propulsive appliance increased even more, expressively different from the age-matched controls. The expression patterns of both IGFs were similar, although IGF-I labelling was stronger. Furthermore, the enhanced expression of both peptides was in parallel with the proliferating cell nuclear antigen (PCNA) positivity, a proliferation cell marker. The modulation of IGF-I and IGF-II expression in the condylar cartilage in response to the propulsive appliance suggests that both peptides are involved in the mandibular adaptation during this therapy. |
| Databáze: | OpenAIRE |
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