Expression of TRAIL and Fas in Primary Hyperparathyroidism
| Autor: | Marlena Brzozowa-Zasada, Romuald Wojnicz, Łukasz Mielańczyk, Jacek Gawrychowski, Oliwia Segiet, Mariusz Deska, Grzegorz Buła |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Pathology medicine.medical_specialty Pathogenesis TNF-Related Apoptosis-Inducing Ligand 03 medical and health sciences 0302 clinical medicine medicine Humans fas Receptor Aged biology Parathyroid neoplasm business.industry Middle Aged medicine.disease Fas receptor Hyperparathyroidism Primary 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Monoclonal biology.protein Immunohistochemistry Surgery Parathyroid gland Antibody business Primary hyperparathyroidism |
| Zdroj: | Journal of investigative surgery : the official journal of the Academy of Surgical Research. 30(4) |
| ISSN: | 1521-0553 |
| Popis: | Differentiating between parathyroid lesions is still difficult and ambiguous. In cases of primary hyperparathyroidism, appropriate and prompt diagnosis is of great importance for effective treatment and follow-up. A great amount of mechanisms contribute to the pathogenesis of primary hyperparathyroidism, such as disturbance in balance between pro- and anti-apoptotic factors. Therefore, we examined whether immunohistochemical expression of apoptotic factors, TNF-related apoptosis-inducing ligand (TRAIL) and Fas, could have clinical utility as a marker of proliferative lesions of parathyroid gland.Parathyroid specimens of 58 consecutive patients who had undertaken surgery due to primary hyperparathyroidism were incubated with purified mouse monoclonal antihuman antibodies: anti-TRAIL and anti-Fas. Staining was considered positive when at least 5% of the cells showed immunoreactivity.The percentage of cells which were positively stained for TRAIL in parathyroid hyperplasia was 9.65%, in parathyroid adenoma 8.31%, and in normal controls 2.24%. Immunoreactivity for TRAIL was detected in 91.89% of parathyroid hyperplasias, 85.71% of parathyroid adenomas, and none in healthy glands. The percentage of cells with a positive reaction to Fas in parathyroid hyperplasia was 8.92%, in parathyroid adenoma 8.09%, and in normal tissue 1.9%. The expression of Fas was found in 94.59% of parathyroid hyperplasias, 90.48% of parathyroid adenomas, and none in healthy glands.In our study, hyperplasias demonstrated the highest expression of TRAIL and Fas, whereas in adenomas it was increased compared to normal tissue, but lower than in hyperplasias. These factors could be an additive tool in the differential diagnosis of parathyroid lesions. |
| Databáze: | OpenAIRE |
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