Systemic lupus erythematosus: disease activity may influence the release of endothelial microparticles?
| Autor: | Walter Batista Cicarini, Bashir A. Lwaleed, Vincente P.C.P. Toledo, Cristina de Mello Gomide Loures, Maria da Gloria Carvalho, Fernanda F. C. Nunes, Claudia Lopes Santoro Neiva, Karine Silvestre Ferreira, Renato Vargas Consoli, Paulo Madureira de Pádua, Luan Carlos Vieira Alves, Edna Afonso Reis |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
030203 arthritis & rheumatology
Adult Lupus erythematosus Systemic blood business.industry Endothelial Cells Hematology General Medicine 030204 cardiovascular system & hematology Middle Aged medicine.disease Disease activity 03 medical and health sciences Young Adult 0302 clinical medicine Cell-Derived Microparticles Healthy volunteers Immunology medicine Humans Lupus Erythematosus Systemic Female Young adult business skin and connective tissue diseases |
| Popis: | To evaluate blood-borne endothelial microparticles (EMPs) in women with SLE and correlated these to disease activity as defined by the SLEDAI-2K score. The study takes cross-sectional design. A total of 90 age-matched women were recruited including: G1 (healthy volunteers, n = 30), G2 (women with SLE and low disease activity (SLEDAI-2K score ≤4; n = 30) and G3 (women with SLE and moderate/high disease activity (SLEDAI-2K score >4; n = 30). Blood was collected in 3.2% sodium citrate. Subsequently, the microparticles were purified by ultracentrifugation and labeled with anti-CD51/61 and anti-Annexin-V antibodies. Quantification and phenotyping were performed using flow cytometry. The number of EMPs was significantly higher in SLE patients compared with controls (P = 0.0178). When SLE patients were stratified according to disease activity, the number of EMPs was significantly increased in women with moderate-to-high disease activity compared with controls (P = 0.0074). We observed a correlation between the number of EMPs and age (r = −0.34; P = 0.0123) and between the number of EMPs and SLEDAI-2K score (r = 0.30; P = 0.04). Our results suggest that the SLE causes increased EMPs release, especially in patients with SLEDAI-2K score greater than 4. Although measurement of the EMPs could be useful in distinguishing patients with SLE from health controls, they have limited value in differentiating between SLE subtypes. |
| Databáze: | OpenAIRE |
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