A Novel Platelet Activating Factor Receptor Antagonist Reduces Cell Infiltration and Expression of Inflammatory Mediators in Mice Exposed to Desiccating Conditions after PRK

Autor: Nicolas G. Bazan, N. Li, Jiucheng He, Isi Esquenazi, Salomon Esquenazi, Haydee E. P. Bazan
Rok vydání: 2009
Předmět:
lcsh:Immunologic diseases. Allergy
Dihydropyridines
Pathology
medicine.medical_specialty
Article Subject
medicine.drug_class
Immunology
Inflammation
Platelet Membrane Glycoproteins
Biology
Photorefractive Keratectomy
Receptors
G-Protein-Coupled
Proinflammatory cytokine
Andrology
Mice
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Immunology and Allergy
Corneal epithelium
Mice
Inbred BALB C
Microscopy
Confocal
Epithelium
Corneal
Antagonist
General Medicine
Receptor antagonist
eye diseases
Disease Models
Animal
Treatment Outcome
medicine.anatomical_structure
030221 ophthalmology & optometry
Cytokines
Increased inflammatory response
Dry Eye Syndromes
Female
sense organs
medicine.symptom
Platelet-activating factor receptor
lcsh:RC581-607
030217 neurology & neurosurgery
Immunostaining
Research Article
Zdroj: Clinical and Developmental Immunology, Vol 2009 (2009)
Clinical and Developmental Immunology
ISSN: 1740-2530
1740-2522
Popis: Purpose. To study the contribution of a novel PAF receptor antagonist LAU-0901 in the modulation of the increased inflammatory response in mice exposed to dessicating conditions (DE) after PRK.Methods. Eighty 13-14 week old female Balb/C mice were used. They were divided into two groups: One group was treated with LAU-0901 topical drops. The other group was treated with vehicle. In each group ten mice served as controls and ten were placed in DE. The other twenty mice underwent bilateral PRK and were divided in two additional groups: ten mice remained under normal conditions (NC) and the other ten were exposed to DE. After 1 week all animals underwent in vivo confocal microscopy, immunostaining and western blotting analysis.Results. Confocal microscopy showed an increased number of reflective structures in the corneal epithelium after PRK and exposure to DE in eyes treated with vehicle as compared to eyes treated with LAU-0901. Significant decrease of COX-2 and Arginase I expression and reduced alpha SMA cells was observed after PRK and exposure to DE in eyes treated with LAU-0901. Discussion: Exposure of mice to a DE after PRK increases the epithelial turnover rate. PAF is involved in the inflammatory cell infiltration and expression of inflammatory cytokines that follow PRK under DE.
Databáze: OpenAIRE
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