α1-Antitrypsin attenuates acute rejection of orthotopic murine lung allografts
| Autor: | Tomoyuki Nakagiri, Fabio Ius, Ann-Kathrin Knöfel, Tobias Goecke, Patrick Zardo, Tobias Welte, Sabine Wrenger, Veronika Grau, Kokilavani Sivaraman, Axel Haverich, Sabina Janciauskiene |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Graft Rejection
congenital hereditary and neonatal diseases and abnormalities Serine Proteinase Inhibitors medicine.medical_treatment Primary Graft Dysfunction Acute allograft rejection Mice Diseases of the respiratory system Immune system medicine Animals Lung transplantation chemistry.chemical_classification Mice Inbred BALB C RC705-779 business.industry Research Mouse orthotopic single lung transplantation model Primary graft dysfunction T helper cell Allografts Flow Cytometry Mice Inbred C57BL CXCL1 Transplantation Disease Models Animal α1 antitrypsin medicine.anatomical_structure chemistry alpha 1-Antitrypsin Acute Disease Immunology Alpha1-antitrypsin Glycoprotein business |
| Zdroj: | Respiratory Research, Vol 22, Iss 1, Pp 1-12 (2021) Respiratory Research |
| Popis: | Background α1-Antitrypsin (AAT) is an acute phase glycoprotein, a multifunctional protein with proteinase inhibitory, anti-inflammatory and cytoprotective properties. Both preclinical and clinical experiences show that the therapy with plasma purified AAT is beneficial for a broad spectrum of inflammatory conditions. The potential effects of AAT therapy have recently been highlighted in lung transplantation (LuTx) as well. Methods We used a murine fully mismatched orthotopic single LuTx model (BALB/CJ as donors and C57BL/6 as recipients). Human AAT preparations (5 mg, n = 10) or vehicle (n = 5) were injected to the recipients subcutaneously prior to and intraperitoneally immediately after the LuTx. No immune suppressive drugs were administered. Three days after the transplantation, the mice were sacrificed, and biological samples were assessed. Results Histological analysis revealed significantly more severe acute rejection in the transplanted lungs of controls than in AAT treated mice (p Conclusion Therapy with AAT suppresses the acute rejection after LuTx in a mouse model. The beneficial effects seem to involve anti-protease and immunomodulatory activities of AAT. |
| Databáze: | OpenAIRE |
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