Role of the Scavenger Receptor CD36 in Accelerated Diabetic Atherosclerosis
| Autor: | Navas Madroñal, Miquel, Castelblanco, Esmeralda, Camacho, Mercedes, Consegal, Marta, Ramírez-Morros, Anna, Sarrias, Maria-Rosa, Perez, Paulina, Alonso, Núria, Galán, María, Mauricio Puente, Dídac, Universitat Autònoma de Barcelona |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
CD36 Antigens Male Vascular smooth muscle CD36 030204 cardiovascular system & hematology Muscle Smooth Vascular lcsh:Chemistry 0302 clinical medicine lcsh:QH301-705.5 Spectroscopy Vascular calcification Medial arterial calcification Receptors Scavenger biology diabetes Chemistry Diabetes Calcinosis General Medicine Middle Aged Flow Cytometry Computer Science Applications Lipoproteins LDL vascular calcification cardiology Female lipids (amino acids peptides and proteins) medicine.symptom scavenger receptor CD36 medicine.medical_specialty Myocytes Smooth Muscle Inflammation Catalysis Article Inorganic Chemistry Diabetes Complications 03 medical and health sciences Diabetes mellitus Internal medicine Diabetes Mellitus medicine Humans Physical and Theoretical Chemistry Scavenger receptor Molecular Biology Aged business.industry Organic Chemistry medicine.disease Atherosclerosis Scavenger receptor CD36 030104 developmental biology Glucose Endocrinology lcsh:Biology (General) lcsh:QD1-999 inflammation Hyperglycemia Unfolded protein response biology.protein atherosclerosis business Calcification Lipoprotein |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona International Journal of Molecular Sciences Volume 21 Issue 19 International Journal of Molecular Sciences, Vol 21, Iss 7360, p 7360 (2020) r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol instname |
| ISSN: | 1422-0067 |
| Popis: | Diabetes mellitus entails increased atherosclerotic burden and medial arterial calcification, but the precise mechanisms are not fully elucidated. We aimed to investigate the implication of CD36 in inflammation and calcification processes orchestrated by vascular smooth muscle cells (VSMCs) under hyperglycemic and atherogenic conditions. We examined the expression of CD36, pro-inflammatory cytokines, endoplasmic reticulum (ER) stress markers, and mineralization-regulating enzymes by RT-PCR in human VSMCs, cultured in a medium containing normal (5 mM) or high glucose (22 mM) for 72 h with or without oxidized low-density lipoprotein (oxLDL) (24 h). The uptake of 1,1&prime dioctadecyl-3,3,3&prime 3-tetramethylindocarbocyanine perchlorate-fluorescently (DiI) labeled oxLDL was quantified by flow cytometry and fluorimetry and calcification assays were performed in VSMC cultured in osteogenic medium and stained by alizarin red. We observed induction in the expression of CD36, cytokines, calcification markers, and ER stress markers under high glucose that was exacerbated by oxLDL. These results were confirmed in carotid plaques from subjects with diabetes versus non-diabetic subjects. Accordingly, the uptake of DiI-labeled oxLDL was increased after exposure to high glucose. The silencing of CD36 reduced the induction of CD36 and the expression of calcification enzymes and mineralization of VSMC. Our results indicate that CD36 signaling is partially involved in hyperglycemia and oxLDL-induced vascular calcification in diabetes. |
| Databáze: | OpenAIRE |
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