Genetic and pathogenic characterization of a Russian subtype 2 PRRSV-1 isolate
| Autor: | A G Yuzhakov, Ivan Trus, Andrei N. Skrylev, Hans Nauwynck, T. V. Grebennikova, Alexei D. Zaberezhny, Alexander M. Mishin, Oleg A. Verkhovsky, Sergei A. Raev, T. I. Aliper |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Swine 040301 veterinary sciences viruses animal diseases Porcine Reproductive and Respiratory Syndrome Virulence Viremia Genome Viral Microbiology Virus Russia 0403 veterinary science Random Allocation 03 medical and health sciences Blood serum Molecular genetics medicine Animals Porcine respiratory and reproductive syndrome virus Amino Acid Sequence Lung Phylogeny General Veterinary biology Strain (chemistry) virus diseases 04 agricultural and veterinary sciences General Medicine Viral Load medicine.disease Porcine reproductive and respiratory syndrome virus biology.organism_classification Virology 030104 developmental biology Sequence Alignment Viral load |
| Zdroj: | Veterinary Microbiology. 211:22-28 |
| ISSN: | 0378-1135 |
| Popis: | Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive failure and respiratory problems. Data about the virulence and pathogenicity of subtype 2 PRRSV-1 strains are limited. The main purposes of this investigation were to characterize the full genome sequence of the subtype 2 PRRSV-1 WestSib13 strain and to compare the pathogenicity with that of the subtype 1 PRRSV-1 Lelystad strain. Comparison of the whole genome sequence of the WestSib13 strain with that of PRRSV-1 prototype strains revealed a 76.2% (subtype 1 Lelystad virus) and 79.0% (subtype 3 Lena virus) identity, respectively The virulence and pathogenicity of the European subtype 2 PRRSV strain WestSib13 and the European subtype 1 PRRSV strain Lelystad were compared in 3-week-old piglets upon inoculation of 10 5.4 TCID 50 of virus. Non-infected animals (control group) as well as animals infected with the Lelystad strain were clinically healthy until 14 days post challenge. In contrast, animals infected with the WestSib13 strain demonstrated dyspnea starting at 3 days post-inoculation (dpi). All piglets in this group died between 5 and 8 dpi. During that period, fever was not observed in WestSib13-infected animals. Viremia was detected in animals from both infected groups starting from 2 dpi. Viral loads in serum and lungs upon euthanasia were significantly higher (3 log 10 ) in the WestSib13-infected than in the LV-infected animals. Taken together, this study provides the full genome sequence and the unusual virological and clinical outcome (high level viremia without fever) of the novel WestSib13 strain. |
| Databáze: | OpenAIRE |
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