Water mediated Dickerson-Drew-type crystal of DNA dodecamer containing 2′-deoxy-5-formyluridine
| Autor: | Yoshihito Ueno, Jiro Kondo, Akira Matsuda, Akio Takénaka, Naoko Karino, M. Tsunoda |
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| Rok vydání: | 2002 |
| Předmět: |
Cations
Divalent Biophysics chemistry.chemical_element Crystal structure Crystallography X-Ray Biochemistry law.invention Divalent Ion Crystal law Molecule Magnesium Crystallization Base Pairing chemistry.chemical_classification Organic Chemistry Water Deoxyuridine Crystallography Dodecameric protein Oligodeoxyribonucleotides chemistry Barium Nucleic Acid Conformation |
| Zdroj: | Biophysical Chemistry. 95:227-233 |
| ISSN: | 0301-4622 |
| DOI: | 10.1016/s0301-4622(01)00259-9 |
| Popis: | To investigate the role of divalent cations in crystal packing, a Dickerson-Drew-type dodecamer with the sequence d(CGCGAATXCGCG), containing 2'-deoxy-5-formyluridine at X, was crystallized under several conditions with Ba(2+) ion instead of Mg(2+) ion. The crystal structure is isomorphous with the original Dickerson-type crystal containing Mg(2+) ion. In the Mg(2+)-free crystals, however, a five-membered ring of water molecules occupies the same position as the magnesium site found in the Mg(2+)-containing crystals, and connects the two duplexes similarly to the hydrated Mg(2+) ion. It has been concluded that the five-membered water molecules can take the place of the hydrated magnesium cation in crystallization. The 5-formyluracil residues form the canonical Watson-Crick pair with the opposite adenine residues. |
| Databáze: | OpenAIRE |
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