Optimized Isolation of Extracellular Vesicles From Various Organic Sources Using Aqueous Two-Phase System
| Autor: | Zeynep Islek, Beyza Mat, Pakize Neslihan Taşlı, Fikrettin Şahin, Hülya Ölmez, Oğuz Kaan Kırbaş, Batuhan Turhan Bozkurt, Dilek Öztürkoğlu, Bihter Ozdemir, Ayla Burcin Asutay |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Biochemistry Extracellular vesicles Article Exocytosis Polyethylene Glycols Extracellular Vesicles 03 medical and health sciences 0302 clinical medicine Animals Humans Parasites Leishmania infantum lcsh:Science Secretion Cells Cultured Multivesicular bodies Multidisciplinary Chemistry lcsh:R Aqueous two-phase system Water Nanobiotechnology Dextrans Plants Isolation (microbiology) 030104 developmental biology 030220 oncology & carcinogenesis Clinical diagnosis Nanoparticles lcsh:Q Biomarkers |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-55477-0 |
| Popis: | From biomarkers to drug carriers, Extracellular Vesicles (EVs) are being used successfully in numerous applications. However, while the subject has been steadily rising in popularity, current methods of isolating EVs are lagging behind, incapable of isolating EVs at a high enough quantity or quality while also requiring expensive, specialized equipment. The “isolation problem” is one of the major obstacles in the field of EV research - and even more so for their potential, widespread use for clinical diagnosis and therapeutic applications. Aqueous Two-Phase Systems (ATPS) has been reported previously as a promising method for isolating EVs quickly and efficiently, and with little contaminants - however, this method has not seen widespread use. In this study, an ATPS-based isolation protocol is used to isolate small EVs from plant, cell culture, and parasite culture sources. Isolated EVs were characterized in surface markers, size, and morphological manner. Additionally, the capacity of ATPS-based EV isolation in removing different contaminants was shown by measuring protein, fatty acid, acid, and phenol red levels of the final isolate. In conclusion, we have shown that EVs originating from different biological sources can be isolated successfully in a cost-effective and user-friendly manner with the use of aqueous two-phase systems. |
| Databáze: | OpenAIRE |
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