Lineage-Restricted Neural Precursors Can Be Isolated from Both the Mouse Neural Tube and Cultured ES Cells
| Autor: | Tahmina Mujtaba, David R. Piper, Mahendra S. Rao, Andrew K. Groves, Mary T. Lucero, Anjali J. Kalyani |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
E-NCAM
Nerve Tissue Proteins Biology self-renewal Nervous System Polymerase Chain Reaction glioblast Mice Neuroblast Gangliosides Precursor cell medicine Animals Neural Cell Adhesion Molecules development Molecular Biology Cells Cultured Neurons Stem Cells fungi Neural tube Cell Differentiation Epithelial Cells Cell Biology Immunohistochemistry ES cells Embryonic stem cell Culture Media Rats Cell biology ErbB Receptors Mice Inbred C57BL Neuroepithelial cell Chemically defined medium medicine.anatomical_structure Spinal Cord Immunology Calcium Neuron Stem cell A2B5 neuroblast Developmental Biology |
| Zdroj: | Developmental Biology. 214(1):113-127 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.1999.9418 |
| Popis: | We have previously identified multipotent neuroepithelial (NEP) stem cells and lineage-restricted, self-renewing precursor cells termed NRPs (neuron-restricted precursors) and GRPs (glial-restricted precursors) present in the developing rat spinal cord (A. Kalyani, K. Hobson, and M. S. Rao, 1997, Dev. Biol. 186, 202–223; M. S. Rao and M. Mayer-Proschel, 1997, Dev. Biol. 188, 48–63; M. Mayer-Proschel, A. J. Kalyani, T. Mujtaba, and M. S. Rao, 1997, Neuron 19, 773–785). We now show that cells identical to rat NEPs, NRPs, and GRPs are present in mouse neural tubes and that immunoselection against cell surface markers E-NCAM and A2B5 can be used to isolate NRPs and GRPs, respectively. Restricted precursors similar to NRPs and GRPs can also be isolated from mouse embryonic stem cells (ES cells). ES cell-derived NRPs are E-NCAM immunoreactive, undergo self-renewal in defined medium, and differentiate into multiple neuronal phenotypes in mass culture. ES cells also generate A2B5-immunoreactive cells that are similar to E9 NEP-cell-derived GRPs and can differentiate into oligodendrocytes and astrocytes. Thus, lineage restricted precursors can be generated in vitro from cultured ES cells and these restricted precursors resemble those derived from mouse neural tubes. These results demonstrate the utility of using ES cells as a source of late embryonic precursor cells. |
| Databáze: | OpenAIRE |
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