Oxacillin- and Mupirocin-ResistantStaphylococcus aureus: In vitroActivity of Silver Sulphadiazine and Cerium Nitrate in Hospital Strains
| Autor: | Marlei Gomes da Silva, R.P. Schuenck, K.R.N. Santos, P. Dadalti, L.S. Fonseca |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Staphylococcus aureus food.ingredient Mupirocin Microbial Sensitivity Tests medicine.disease_cause Polymerase Chain Reaction Microbiology Agar dilution chemistry.chemical_compound Minimum inhibitory concentration food Bacterial Proteins Drug Resistance Bacterial medicine Humans Penicillin-Binding Proteins Agar Pharmacology (medical) Agar diffusion test DNA Primers Oxacillin Pharmacology Cross Infection business.industry Cerium Staphylococcal Infections Silver Sulfadiazine In vitro Cerium nitrate Infectious Diseases Oncology chemistry Anti-Infective Agents Local lipids (amino acids peptides and proteins) business Brazil |
| Zdroj: | Journal of Chemotherapy. 16:453-458 |
| ISSN: | 1973-9478 1120-009X |
| DOI: | 10.1179/joc.2004.16.5.453 |
| Popis: | Nasal carriage is an important reservoir of oxacillin-resistant Staphylococcus aureus (ORSA). Mupirocin is a topical drug used to remove S. aureus from nares. However, isolates resistant to mupirocin have been reported all over the world. Silver sulphadiazine (SSD) is a topical agent, which when associated with cerium nitrate (CN), has been shown to be useful in the treatment of burn infections and could be an alternative drug for patient decolonization. Susceptibility to oxacillin in 203 S. aureus isolates was evaluated by the agar diffusion test, while the agar diffusion and agar dilution methods were used for mupirocin. A PCR-multiplex method was performed to detect the mecA and ileS-2 genes. Minimum inhibitory concentration (MICs) to SSD and CN, used alone or in association, were determined by the agar dilution method. One hundred and sixty-three (80.3%) strains were oxacillin-resistant, and 37 (18.2%) were mupirocin resistant. The MIC of SSD alone or in association with CN was 64 microg/mL, while for CN alone was 2048 microg/mL for all isolates. SSD presented anti-staphylococcal activity at concentrations (64 microg/mL) much lower than those commonly used in commercial preparations (10 mg/g) and had good activity against mupirocin-resistant strains, showing that this drug could be used for nasal decolonization in ORSA carries. |
| Databáze: | OpenAIRE |
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