Active Hyaluronidase 2 Expression in the Granulation Tissue Formed in the Healing Process of Equine Superficial Digital Flexor Tendonitis
| Autor: | Yoshinori Kasashima, Yohei Yuda, Kan Sato, Katsuhiko Arai, Atsutoshi Kuwano, Shunji Hattori |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty Hyaluronoglucosaminidase Tendonitis In situ hybridization Gene Expression Regulation Enzymologic Hyaluronidase Forelimb medicine Animals Zymography Horses Frozen section procedure General Veterinary Chemistry Granulation tissue Anatomy musculoskeletal system Up-Regulation Tendon medicine.anatomical_structure Tendinopathy Granulation Tissue Horse Diseases Wound healing medicine.drug |
| Zdroj: | Journal of Veterinary Medical Science. 75:219-223 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.12-0318 |
| Popis: | Upregulation of hyaluronidase 2 (HYAL2), one of somatic hyaluronidase (HAase), was demonstrated in granulation tissue during the healing of equine superficial digital flexor tendon injuries. The activity of HAase was assessed by hyaluronan (HA)-containing gel zymography and in situ zymography using frozen sections obtained from normal and injured tendon tissues. Elevated HAase activity was identified in the extract from the tendinopathic tissues, with lower levels of the activity in normal tendons. In situ zymography using fluorescently-labeled HA demonstrated HAase activity in the granulation tissue formed in the injured region. In addition, in situ hybridization analysis indicated that fibroblastic cells in the granulation tissue of the injured tendon actively expressed HYAL2 but not HYAL1. Quantitative RT-PCR further confirmed a higher level of amplicons corresponding to HYAL2 in tendonitis-derived samples. These results suggest that elevated HYAL2 activity in the granulation tissue could participate in controlling the healing process in equine tendonitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|